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VEGF ELISA 试剂盒

Name: VEGF ELISA 试剂盒
SKU: ABIN6719756
Availability: OutOfStock

This 人 VEGF ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 VEGF.

ELISA

1 image

产品编号 ABIN6719756

Supplier Product No.: koa0329

发货至: 中国

Quick Overview for VEGF ELISA 试剂盒 (ABIN6719756)

抗原

See all VEGF ELISA试剂盒

VEGF (Vascular Endothelial Growth Factor (VEGF))

适用

人

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

31.2 pg/mL - 2000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum

Discover our top product VEGF ELISA Kit

最低检测浓度

31.2 pg/mL

Supplier Product No.

koa0329

Supplier

Rockland

原理

Human VEGF Sandwich ELISA Kit for Quantitative Detection

品牌

AccuSignal™

Analytical Method

Quantitative

特异性

Production: Natural and recombinant human VEGF. There is no detectable cross-reactivity with other relevant proteins.

灵敏度

< 1 pg/mL

组件
- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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应用备注

Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human VEGF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. We recommend that each human VEGF standard solution and each sample is measured in duplicate.

说明

Standard: Expression system for standard: sf21, Immunogen sequence: A27-R191
Detection Range: 31.2 pg/ml - 2000 pg/ml (serum, plasma), 15.6 pg/ml - 1000 pg/ml (cell culture supernates)

样本量

100 μL

板类型

Pre-coated

限制

仅限研究用
储存条件

4 °C,-20 °C

储存方法

Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

有效期

12 months
抗原 See all VEGF ELISA试剂盒

VEGF (Vascular Endothelial Growth Factor (VEGF))

别名

VEGF

背景

Synonyms: MGC70609, MVCD1, Vascular endothelial growth factor, Vascular endothelial growth factor A, Vascular permeability factor, VEGF A, VEGFA, VPF
Background: Vascular permeability factor/vascular endothelial growth factor(VPF/VEGF), a potent cytokine expressed by most malignant tumors, has critical roles in vasculogenesis and both physiological and pathological angiogenesis. VEGF produced by tumor cells potently stimulates endothelial cell proliferation and angiogenesis and plays a key role in the pathophysiology of several neoplasias. VEGF may also play a pivotal role in mediating the development and progression of diabetic retinopathy. VEGF, a major regulator of angiogenesis, binds to two receptor tyrosine kinases, KDR/Flk-1 and Flt-1. The VEGF gene is mapped by fluorescence in situ hybridization to chromosome 6p12.

基因ID

7422

NCBI登录号

NP_001020537

UniProt

P15692