VEGF ELISA 试剂盒
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北京 101111
Quick Overview for VEGF ELISA 试剂盒 (ABIN411369)
抗原
See all VEGF ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 31.2 pg/mL
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原理
- Sandwich Human VEGF ELISA Kit to quantitate Human VEGFA in cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: A27-R191Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 1 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Vascular Endothelial Growth Factor (VEGF) ELISA Kit
VEGF 适用: 大鼠 AA 27-190 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Vascular Endothelial Growth Factor (VEGF) ELISA KitVEGF 适用: 小鼠 AA 27-190 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Vascular Endothelial Growth Factor (VEGF) ELISA KitVEGF 适用: Pig Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Vascular Endothelial Growth Factor (VEGF) IQ-ELISA KitVEGF 适用: 人 qPCR Sandwich ELISA Cell Culture Supernatant, Plasma, Serum
Vascular Endothelial Growth Factor (VEGF) ELISA KitVEGF 适用: 犬 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Vascular Endothelial Growth Factor (VEGF) ELISA KitVEGF 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Vascular Endothelial Growth Factor (VEGF) ELISA KitVEGF 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Vascular Endothelial Growth Factor (VEGF) ELISA KitVEGF 适用: 兔 Colorimetric Sandwich ELISA 1.563 pg/mL - 100 pg/mL Plasma, Serum, Tissue Homogenate
Vascular Endothelial Growth Factor (VEGF) ELISA KitVEGF 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Vascular Endothelial Growth Factor (VEGF) ELISA KitVEGF 适用: 小鼠 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 9.6%, 7.9%, 9.6%
Inter-Assay Precision (CV%): 7.8%, 9.6%, 7.8% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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: "DHX32 Promotes Angiogenesis in Colorectal Cancer Through Augmenting β-catenin Signaling to Induce Expression of VEGFA." in: EBioMedicine, Vol. 18, pp. 62-72, (2018) (PubMed).
: "The expression and underlying angiogenesis effect of DPC4 and VEGF on the progression of cervical carcinoma." in: Oncology letters, Vol. 15, Issue 2, pp. 2534-2540, (2018) (PubMed).
: "Histone demethylase KDM4D promotes gastrointestinal stromal tumor progression through HIF1β/VEGFA signalling." in: Molecular cancer, Vol. 17, Issue 1, pp. 107, (2018) (PubMed).
: "Elevated expression levels of serum insulin-like growth factor-1, tumor necrosis factor-α and vascular endothelial growth factor 165 might exacerbate type 2 diabetic nephropathy." in: Journal of diabetes investigation, Vol. 8, Issue 1, pp. 108-114, (2017) (PubMed).
: "5-Aminolaevulinic Acid-Based Photodynamic Therapy Restrains Pathological Hyperplasia of Fibroblasts." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 23, pp. 46-56, (2017) (PubMed).
: "Growth Factor Variation in Two Types of Autologous Platelet Biomaterials: PRP Versus PRF." in: Indian journal of hematology & blood transfusion : an official journal of Indian Society of Hematology and Blood Transfusion, Vol. 33, Issue 2, pp. 288-292, (2017) (PubMed).
: "Long non-coding RNA HOTAIR enhances angiogenesis by induction of VEGFA expression in glioma cells and transmission to endothelial cells via glioma cell derived-extracellular vesicles." in: American journal of translational research, Vol. 9, Issue 11, pp. 5012-5021, (2017) (PubMed).
: "Wogonin inhibits multiple myeloma-stimulated angiogenesis via c-Myc/VHL/HIF-1α signaling axis." in: Oncotarget, Vol. 7, Issue 5, pp. 5715-27, (2016) (PubMed).
: "Genetic Risk Factors for Psoriasis in Turkish Population: -1540 C/A, -1512 Ins18, and +405 C/G Polymorphisms within the Vascular Endothelial Growth Factor Gene." in: Annals of dermatology, Vol. 28, Issue 1, pp. 30-9, (2016) (PubMed).
: "Serum level of vascular endothelial growth factor decreased in chronic ketamine abusers." in: Drug and alcohol dependence, Vol. 152, pp. 57-61, (2015) (PubMed).
: "?-tocopherol supplementation prevents lead acetate and hypoxia-induced hepatic dysfunction." in: Indian journal of pharmacology, Vol. 47, Issue 3, pp. 285-91, (2015) (PubMed).
: "Hypoxia-inducible factor-1? protects cervical carcinoma cells from apoptosis induced by radiation via modulation of vascular endothelial growth factor and p53 under hypoxia." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 21, pp. 318-25, (2015) (PubMed).
: "Overexpression of VEGF183 promotes murine breast cancer cell proliferation in vitro and induces dilated intratumoral microvessels." in: Tumour biology, Vol. 36, Issue 5, pp. 3871-80, (2015) (PubMed).
: "[Corrigendum] Overexpression of the chimeric plasmin-resistant VEGF165/VEGF183 (132-158) protein in murine breast cancer induces distinct vascular patterning adjacent to tumors and retarded tumor ..." in: Molecular medicine reports, Vol. 11, Issue 5, pp. 4010, (2015) (PubMed).
: "Nuclear factor of activated T cells 5 maintained by Hotair suppression of miR-568 upregulates S100 calcium binding protein A4 to promote breast cancer metastasis." in: Breast cancer research : BCR, Vol. 16, Issue 5, pp. 454, (2015) (PubMed).
: "Epigallocatechin-3-gallate inhibits nicotine-induced migration and invasion by the suppression of angiogenesis and epithelial-mesenchymal transition in non-small cell lung cancer cells." in: Oncology reports, Vol. 33, Issue 6, pp. 2972-80, (2015) (PubMed).
: "Human umbilical cord mesenchymal stem cells promote peripheral nerve repair via paracrine mechanisms." in: Neural regeneration research, Vol. 10, Issue 4, pp. 651-8, (2015) (PubMed).
: "Important of Angiopoietic System in Evaluation of Endothelial Damage in Children with Crimean-Congo Hemorrhagic Fever." in: The Pediatric infectious disease journal, Vol. 34, Issue 8, pp. e200-5, (2015) (PubMed).
: "High-mobility group box 1 expression and lymph node metastasis in intrahepatic cholangiocarcinoma." in: World journal of gastroenterology : WJG, Vol. 21, Issue 11, pp. 3256-65, (2015) (PubMed).
: "Epigallocatechin-3-gallate attenuates lipopolysaccharide-induced mastitis in rats via suppressing MAPK mediated inflammatory responses and oxidative stress." in: International immunopharmacology, Vol. 26, Issue 1, pp. 147-52, (2015) (PubMed).
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- VEGF (Vascular Endothelial Growth Factor (VEGF))
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背景
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Background: Vascular permeability factor/vascular endothelial growth factor (VPF/VEGF), a potent cytokine expressed by most malignant tumors, has critical roles in vasculogenesis and both physiological and pathological angiogenesis.VEGF produced by tumor cells potently stimulates endothelial cell proliferation and angiogenesis and plays a key role in the pathophysiology of several neoplasias. VEGF may also play a pivotal role in mediating the development and progression of diabetic retinopathy.VEGF, a major regulator of angiogenesis, binds to two receptor tyrosine kinases, KDR/Flk-1 and Flt-1. The VEGF gene is mapped by fluorescence in situ hybridization to chromosome 6p12.
Gene Full Name: vascular endothelial growth factor A
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基因ID
- 7422
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UniProt
- P15692
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