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MMP12 ELISA 试剂盒

This 人 MMP12 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 MMP12.
Rockland
产品编号 ABIN6719730
Supplier Product No.: koa0581
发货至: 中国

Quick Overview for MMP12 ELISA 试剂盒 (ABIN6719730)

抗原

See all MMP12 ELISA试剂盒
MMP12 (Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12))

适用

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检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

62.5 pg/mL - 4000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Plasma (heparin), Serum
  • 最低检测浓度

    62.5 pg/mL

    Supplier Product No.

    koa0581

    Supplier

    Rockland

    原理

    Human MMP-12 Sandwich ELISA Kit for Quantitative Detection

    品牌

    AccuSignal™

    Analytical Method

    Quantitative

    特异性

    Production: Natural and recombinant human MMP-12. There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    < 10 pg/mL

    组件

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • 应用备注

    ELISA_Dilution: 62.5pg/mL-4000pg/mL

    Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human MMP-12 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin) to each empty well. It is recommended that each human MMP-12 standard solution and each sample be measured in duplicate.

    说明

    Standard: Expression system for standard: NSO, Immunogen sequence: L17-C470

    样本量

    100 μL

    板类型

    Pre-coated

    限制

    仅限研究用
  • 储存条件

    4 °C,-20 °C

    储存方法

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    有效期

    12 months
  • 抗原 See all MMP12 ELISA试剂盒

    MMP12 (Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12))

    别名

    MMP-12

    背景

    Synonyms: EC 3.4.24.65, HME, Macrophage elastase, Macrophage metalloelastase, Macrophage metaloelastase, Matrix metallopeptidase 12 (macrophage elastase), Matrix metalloprotease 12, Matrix metalloproteinase-12, MGC138506, MME, MMP 12, MMP-12, Mmp12, MMP12_HUMAN

    Background: Matrix metalloproteinase-12(MMP12), also known as MME or ME, is an enzyme that in humans is encoded by the MMP12 gene. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.2. It is thought that the protein encoded by this gene is cleaved at both ends to yield the active enzyme, but this processing has not been fully described. The enzyme degrades soluble and insoluble elastin. It may play a role in aneurysm formation and studies in mice suggest a role in the development of emphysema. This gene may involved in tissue injury and remodeling.

    基因ID

    4321

    NCBI登录号

    NP_002417

    UniProt

    P39900
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