WISP1 ELISA 试剂盒
Quick Overview for WISP1 ELISA 试剂盒 (ABIN6719711)
抗原
See all WISP1 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 31.2 pg/mL
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Supplier Product No.
- koa0694
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Supplier
- Rockland
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原理
- Mouse WISP1 - CCN4 Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Production: Natural and recombinant mouse WISP1. There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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WNT1 Inducible Signaling Pathway Protein 1 (WISP1) ELISA Kit
WISP1 适用: 人 Colorimetric Sandwich ELISA 78.13 pg/mL - 5000 pg/mL Cell Culture Supernatant, Plasma, Serum
WNT1 Inducible Signaling Pathway Protein 1 (WISP1) ELISA KitWISP1 适用: 小鼠 Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Plasma, Serum, Tissue Homogenate
WNT1 Inducible Signaling Pathway Protein 1 (WISP1) ELISA KitWISP1 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
WNT1 Inducible Signaling Pathway Protein 1 (WISP1) ELISA KitWISP1 适用: 大鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
WNT1 Inducible Signaling Pathway Protein 1 (WISP1) ELISA KitWISP1 适用: 小鼠 AA 23-367 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
WNT1 Inducible Signaling Pathway Protein 1 (WISP1) ELISA KitWISP1 适用: 人 AA 23-367 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
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应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse WISP1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse WISP1 standard solution and each sample be measured in duplicate.
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说明
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Standard: Expression system for standard: NSO, Immunogen sequence: T23-N367
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样本量
- 100 μL
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))
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别名
- WISP1/CCN4
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背景
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Synonyms: CCN family member 4, CCN4, WISP1, WISP1c, WISP1i, WISP1tc, Wnt 1 inducible signaling pathway protein 1, Wnt-1 induced secreted protein, WNT1 induced secreted protein 1, WNT1 inducible signaling pathway protein 1, WNT1 inducible-signaling pathway protein 1
Background: WISP1(WNT1-Inducible Signaling Pathway Protein 1), also known as CCN4, is a matricellular protein that in humans is encoded by the WISP1 gene. WISP1 is induced by WNT1 and belongs to the CCN family, which includes connective tissue growth factor, cysteine-rich-61, and nephroblastoma overexpressed. By use of radiation hybrid mapping panels, the WISP1 was mapped to chromosome 8q24.1-q24.3, roughly 4 Mb distal to MYC. It found that 2 distinct systems demonstrated that WISP induction was associated with expression of WNT1. WISP1 genomic DNA was amplified in colon cancer cell lines and in human colon tumors, and its RNA was overexpressed in 84 % of tumors examined compared with patient-matched normal mucosa.
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基因ID
- 22402
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NCBI登录号
- NP_061353
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UniProt
- O54775
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途径
- WNT signaling, Growth Factor Binding
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