TNFSF18 ELISA 试剂盒
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北京 101111
Quick Overview for TNFSF18 ELISA 试剂盒 (ABIN6719708)
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See all TNFSF18 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 31.2 pg/mL
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Supplier Product No.
- koa0782
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Supplier
- Rockland
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原理
- Mouse TNFSF18 - GITRL Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Production: Natural and recombinant mouse TNFSF18. There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse TNFSF18 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse TNFSF18 standard solution and each sample be measured in duplicate.
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说明
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Standard: Expression system for standard: NSO, Immunogen sequence: T47-S173
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样本量
- 100 μL
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- TNFSF18 (Tumor Necrosis Factor (Ligand) Superfamily, Member 18 (TNFSF18))
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别名
- TNFSF18/GITRL
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背景
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Synonyms: Activation inducible TNF related ligand, Activation-inducible TNF-related ligand, AITRL, GITRL, Glucocorticoid induced TNF related ligand, Glucocorticoid induced TNFR related protein ligand, Glucocorticoid-induced TNF-related ligand, hGITRL, MGC138237, TL6, TNF18_HUMAN, TNFSF18, Tumor necrosis factor (ligand) superfamily member 18, Tumor necrosis factor ligand superfamily member 18
Background: Tumor necrosis factor ligand superfamily member 18, also known as AITRL or GITRL, is a protein that in humans is encoded by the TNFSF18 gene. The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor(TNF) ligand family. It is mapped to 1q25.1. TNFSF18 is a ligand for receptor TNFRSF18/AITR/GITR. It has been shown to modulate T lymphocyte survival in peripheral tissues. This cytokine is also found to be expressed in endothelial cells, and is thought to be important for interaction between T lymphocytes and endothelial cells. TNFSF18-dependent modulation of tryptophan catabolism may represent an important mechanism of action of glucocorticoids, both physiologically and therapeutically.
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基因ID
- 240873
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NCBI登录号
- NP_899247
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UniProt
- Q7TS55
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