TNFSF18 ELISA 试剂盒

Tissue Specificity: Detected in immature and mature dendritic cells and in macrophages (at protein level). Detected in spleen, lung, heart, thymus, monocytes, macrophages, B-cells and dendritic cells. .

Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse TNFSF18 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse TNFSF18 standard solution and each sample be measured in duplicate.

• Sample 1: n=16, Mean(pg/ml): 183, Standard deviation: 7.7, CV(%): 4.2
• Sample 2: n=16, Mean(pg/ml): 764, Standard deviation: 35.2, CV(%): 4.6
• Sample 3: n=16, Mean(pg/ml): 1360, Standard deviation: 51.7, CV(%): 3.8,
• Sample 1: n=24, Mean(pg/ml): 212, Standard deviation: 12.1, CV(%): 5.7
• Sample 2: n=24, Mean(pg/ml): 862, Standard deviation: 53.4, CV(%): 6.2
• Sample 3: n=24, Mean(pg/ml): 1505, Standard deviation: 72.2, CV(%): 4.8

Protein Function: Cytokine that binds to TNFRSF18/AITR/GITR (PubMed:14521928, PubMed:14647196). Regulates T-cell responses (PubMed:14647196). Can function as costimulator and lower the threshold for T-cell activation and T-cell proliferation (PubMed:14608036, PubMed:15128759). Important for interactions between activated T-lymphocytes and endothelial cells. Mediates activation of NF-kappa-B (PubMed:14521928, PubMed:14647196, PubMed:18178614). Triggers increased phosphorylation of STAT1 and up-regulates expression of VCAM1 and ICAM1 (By similarity). Promotes leukocyte adhesion to endothelial cells (PubMed:23892569). Regulates migration of monocytes from the splenic reservoir to sites of inflammation (PubMed:24107315). .

Background: Tumor necrosis factor ligand superfamily member 18, also known as AITRL or GITRL, is a protein that in humans is encoded by the TNFSF18 gene. The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor(TNF) ligand family. It is mapped to 1q25.1. TNFSF18 is a ligand for receptor TNFRSF18/AITR/GITR. It has been shown to modulate T lymphocyte survival in peripheral tissues. This cytokine is also found to be expressed in endothelial cells, and is thought to be important for interaction between T lymphocytes and endothelial cells. TNFSF18-dependent modulation of tryptophan catabolism may represent an important mechanism of action of glucocorticoids, both physiologically and therapeutically.

Synonyms: Tumor necrosis factor ligand superfamily member 18,GITR ligand,GITRL,Glucocorticoid-induced TNF-related ligand,Tnfsf18,Gitrl,

Full Gene Name: Tumor necrosis factor ligand superfamily member 18