MICA ELISA 试剂盒
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Quick Overview for MICA ELISA 试剂盒 (ABIN6719703)
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See all MICA ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 62.5 pg/mL
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Supplier Product No.
- koa0483
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Supplier
- Rockland
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原理
- Human MICA Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: E24-W307 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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MHC Class I Polypeptide-Related Sequence A (MICA) ELISA Kit
MICA 适用: 人 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
MHC Class I Polypeptide-Related Sequence A (MICA) ELISA KitMICA 适用: 人 AA 24-307 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Cell Lysate, Serum
MHC Class I Polypeptide-Related Sequence A (MICA) ELISA KitMICA 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
MHC Class I Polypeptide-Related Sequence A (MICA) ELISA KitMICA 适用: 人 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Cell Samples, Tissue Lysate
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说明
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Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human MICA standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, cell lysates, tissue lysates or serum to each empty well. It is recommended that each human MICA standard solution and each sample be measured in duplicate.
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实验精密度
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Intra-Assay Precision (CV%): 7.4%, 7.2%, 7.2%
Inter-Assay Precision (CV%): 8.5%, 8.2%, 7.7% -
限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- MICA (MHC Class I Polypeptide-Related Sequence A (MICA))
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别名
- MICA
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背景
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Background: MHC class I polypeptide-related sequence A is a protein that in humans is encoded by the MICA gene.1 The MICA gene encodes a 383-amino acid polypeptide with a predicted mass of 43 kD.The MICA and MICB genes occur in a 200-kb region spanning the TNFA and TNFB cluster at 6p21.3.2 MICA and the closely related MICB were recognized by intestinal epithelial T cells expressing diverse V-delta-1 gamma/delta TCRs.3 The MICA protein product is expressed on the cell surface, although unlike canonical class I molecules does not seem to associate with beta-2-microglobulin. It is further distinguished by its unusual exon-intron organization and preferential expression in fibroblasts and epithelial cells. It is thought that MICA functions as a stress-induced antigen that is broadly recognized by NK cells, NKT cells, and most of the subtypes of T cells. MICA and other members of this family may have been selected for specialized functions that are either ancient or derived from those of typical MHC class I genes, in analogy to some of the nonclassic mouse H-2 genes.
Gene Full Name: MHC class I polypeptide-related sequence A
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基因ID
- 100507436
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NCBI登录号
- NP_000238
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UniProt
- Q29983
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途径
- Activation of Innate immune Response, Transition Metal Ion Homeostasis, Human Leukocyte Antigen (HLA) in Adaptive Immune Response
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