Kallikrein 8 ELISA 试剂盒
Quick Overview for Kallikrein 8 ELISA 试剂盒 (ABIN6719701)
抗原
See all Kallikrein 8 (KLK8) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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Supplier Product No.
- koa0489
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Supplier
- Rockland
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原理
- Human KLK8 - Kallikrein-8 Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Production: Natural and recombinant human KLK8. There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human KLK8 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, cell lysates, tissue homogenates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human KLK8 standard solution and each sample be measured in duplicate.
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说明
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Standard: Expression system for standard: NSO, Immunogen sequence: Q29-G260
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样本量
- 100 μL
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- Kallikrein 8 (KLK8)
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别名
- KLK8/Kallikrein-8
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背景
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Synonyms: Brain serine protease 1, BSP1, hK8, HNP, HPN, Kallikrein 8 (neuropsin/ovasin), Kallikrein related peptidase 8, KLK8 protein type 1, KLK8 protein type 2, KLK8_HUMAN, Neuropsin, Neuropsin type 1, Neuropsin type 2, NP, NRPN, Ovasin, PRO322, Protease serine 19, PRSS 19, Serine protease 19, Serine protease kallikrein, Serine protease kallikrein/ovasin/neuropsin, Serine protease TADG 14, TADG14, Tumor associated differentially expressed gene 14, Tumor associated differentially expressed gene 14 protein
Background: Kallikrein-8 is a protein that in humans is encoded by the KLK8 gene. Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Alternate splicing of this gene results in four transcript variants encoding four different isoforms. The isoforms exhibit distinct patterns of expression that suggest roles in brain plasticity and ovarian cancer.
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基因ID
- 11202
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NCBI登录号
- NP_001268360
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UniProt
- O60259
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途径
- Complement System
抗原 See all Kallikrein 8 (KLK8) ELISA试剂盒
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