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VEGFA IQ-ELISA 试剂盒

VEGFA 适用: 大鼠 qPCR Sandwich ELISA Cell Culture Supernatant, Plasma, Serum
产品编号 ABIN6385021
发货至: 中国
  • 抗原 See all VEGFA IQ-ELISA Kits
    VEGFA (Vascular Endothelial Growth Factor A (VEGFA))
    适用
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    大鼠
    检测方法
    qPCR
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Rat Immunoquantitative (PCR-Based) VEGF-A ELISA Kit for cell culture supernatants, plasma, and serum samples.
    样品类型
    Cell Culture Supernatant, Plasma, Serum
    Analytical Method
    Semi-Quantitative
    灵敏度
    0.2 pg/mL
    产品特性
    IQ-ELISAs employ specific capture antibodies coated on a 96-well PCR plate. Standards and samples are pipetted into the wells, the target protein in the standards and samples binds to the immobilized antibody. The wells are washed and the detection affinity reagent is added to the wells where it binds to any captured antigen. The wells are washed, and primers and PCR master mix are added to each well. The plate is placed into a real time PCR instrument for cycling and measurement of DNA amplification. The cycle number where amplification is detected is proportional to the amount of affinity detection reagent that bound to captured antigen in each well.
    组件
    • VEGF-A Microplate (Item A): 96 well PCR plate coated with anti-Rat VEGF-A
    • Wash Buffer I Concentrate (20x) (Item B): 25 ml of 20x concentrated solution
    • Standards (Item C): 2 vials of recombinant Rat VEGF-A
    • Assay Diluent A (Item D): 30 ml diluent buffer, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent
    • Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent
    • Detection Affinity Reagent for VEGF-A (Item F): 2 vials of a 4x concentrated solution of anti-Rat VEGF-A affinity reagent
    • IQELISA Detection Reagent (Item G): 1.4ml of a 10x concentrated stock
    • Primer Solution (Item I): 1.7 ml vial
    • PCR Master Mix (Item J): 1.2 ml vial
    • PCR Preparation buffer (Item K): 1ml vial of 10x concentrated buffer
    • Final Wash Buffer (Item L): 10ml vial of 10x concentrated buffer
    试剂未包括
    • Real-time PCR instrument, Bio-Rad recommended
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • 100 mL and 1 liter graduated cylinders
    • Absorbent paper
    • Distilled or deionized water
    • Log-log graph paper or computer and software for data analysis
    • Tubes to prepare standard or sample dilutions
    • Heating block or water bath capable of 80°C
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  • 应用备注
    The Immuno-Quantitative ELISA (IQELISA) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. This results in an assay that is simultaneously familiar and cutting edge and enables the use of only 1/10th the sample volume while also providing 10x more sensitivity than a traditional ELISA.
    说明

    The Immuno-Quantitative ELISA (IQELISA™) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. Also called immuno-PCR, this detection platform results in an assay that is simultaneously familiar and cutting edge. Compared to traditional ELISA, IQELISA™ enables the use of only 1/10th the sample volume while also providing 10x more sensitivity.

    样本量
    25 µL
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed
    2. Add 25 μL standard or sample to each well. Incubate for 2.5 hours at room temperature or overnight at 4°C
    3. Add 25 μL detection affinity reagent to each well. Incubate 1 hour at room temperature
    4. Add 25μL of IQELISA Detection Reagent to each well. Incubate 1 hour
    5. Add 15μL Primer solution 10μL of PCR master mix to each well
    6. Run real-time PCR
    限制
    仅限研究用
  • 储存条件
    4 °C,-20 °C,-80 °C
    储存方法
    May be stored for up to 6 months at 2° to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened PCR plate or reagents may be stored for up to 1 month at 2° to 8°C. Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.
    有效期
    6 months
  • 抗原 See all VEGFA IQ-ELISA Kits
    VEGFA (Vascular Endothelial Growth Factor A (VEGFA))
    别名
    VEGF-A (VEGFA 产品)
    别名
    MVCD1 IQ-ELISA Kit, VEGF IQ-ELISA Kit, VPF IQ-ELISA Kit, Vegf IQ-ELISA Kit, Vegf120 IQ-ELISA Kit, Vegf164 IQ-ELISA Kit, Vegf188 IQ-ELISA Kit, Vpf IQ-ELISA Kit, vegf IQ-ELISA Kit, vegfa IQ-ELISA Kit, wu:fj82c06 IQ-ELISA Kit, VEGF-A IQ-ELISA Kit, VEGF164 IQ-ELISA Kit, eVEGF120 IQ-ELISA Kit, eVEGF164 IQ-ELISA Kit, vegf-a IQ-ELISA Kit, vpf IQ-ELISA Kit, vefg IQ-ELISA Kit, si:dkey-14d8.3 IQ-ELISA Kit, wu:fd42e02 IQ-ELISA Kit, vascular endothelial growth factor A IQ-ELISA Kit, vascular endothelial growth factor Aa IQ-ELISA Kit, vascular endothelial growth factor A L homeolog IQ-ELISA Kit, vascular endothelial growth factor Ab IQ-ELISA Kit, VEGFA IQ-ELISA Kit, Vegfa IQ-ELISA Kit, vegfaa IQ-ELISA Kit, vegfa.L IQ-ELISA Kit, vegfa IQ-ELISA Kit, vegfab IQ-ELISA Kit
    基因ID
    83785
    UniProt
    P16612
    途径
    RTK signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
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