HSP27 ELISA 试剂盒
Quick Overview for HSP27 ELISA 试剂盒 (ABIN625460)
抗原
See all HSP27 (HSPB1) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.15 ng/mL
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原理
- Human HSP27 ELISA Kit for cell culture supernatants, plasma, and serum samples.
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Analytical Method
- Quantitative
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特异性
- This ELISA antibody pair detect human HSP27. Other species not determined.
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灵敏度
- 150 pg/mL
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产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Heat Shock 27kDa Protein 1 (HSPB1) ELISA Kit
HSPB1 适用: 大鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Heat Shock 27kDa Protein 1 (HSPB1) ELISA KitHSPB1 适用: 人 AA 2-205 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum, Tissue Homogenate
Heat Shock 27kDa Protein 1 (HSPB1) ELISA KitHSPB1 适用: 小鼠 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Heat Shock 27kDa Protein 1 (HSPB1) ELISA KitHSPB1 适用: 大鼠 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Heat Shock 27kDa Protein 1 (HSPB1) ELISA KitHSPB1 适用: Cow Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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应用备注
- Recommended Dilution for serum and plasma samples3 - 30 fold
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
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有效期
- 6 months
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- HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))
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别名
- HSP27
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背景
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Gene Names: HSPB1 HSP27 HSP28
Protein names: Heat shock protein beta-1 (HspB1) (28 kDa heat shock protein) (Estrogen-regulated 24 kDa protein) (Heat shock 27 kDa protein) (HSP 27) (Stress-responsive protein 27) (SRP27)
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基因ID
- 3315
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UniProt
- P04792
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途径
- MAPK Pathway, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling
抗原 See all HSP27 (HSPB1) ELISA试剂盒
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