MADCAM1 ELISA 试剂盒
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Quick Overview for MADCAM1 ELISA 试剂盒 (ABIN625416)
抗原
See all MADCAM1 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 40.3 pg/mL
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原理
- Mouse MAdCAM-1 ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
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Analytical Method
- Quantitative
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特异性
- This ELISA kit shows no cross-reactivity with the following cytokines tested: mouse 4-1BB (TNFRSF9/CD137), Ace, ALK-1, Cardiotrophin-1 (CT1), CD27 (TNFRSF7), CD32a, CD40 Ligand (TNFSF5), CTLA-4 (CD152), Decorin, DKK-1, Dtk, Endoglin (CD105), Flt-3 Ligand, Galectin-1, Galectin-3, Gas 1, Gas 6, GITR Ligand (TNFSF18), HAI-1, HGFR, IL-1 R4 (ST2), IL-3 R beta, IL-9, JAM-A (CD321/F11R), Leptin R, L-Selectin (CD62L), Lymphotactin (XCL1), MFG-E8, MIP-3 beta (CCL19), Neprilysin, Pentraxin-3 (TSG-14), RAGE, TACI (TNFRSF13B), TREM-1, TROY (TNFRSF19), TSLP, TWEAK R (TNFRSF12), VEGFR1, VEGFR3.
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产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate< div class='ty-product-feature'>< div class='ty-product-feature__label'>Other Materials Required< div class='ty-product-feature__value'>
- Distilled or deionized water
- Precision pipettes to deliver 2 μl to 1 μl volumes
- Adjustable 1-25 μl pipettes for reagent preparation
- 100 μl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate< div class='ty-product-feature'>< div class='ty-product-feature__label'>Other Materials Required< div class='ty-product-feature__value'>
- Distilled or deionized water
- Precision pipettes to deliver 2 μl to 1 μl volumes
- Adjustable 1-25 μl pipettes for reagent preparation
- 100 μl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
-
试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1) ELISA Kit
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Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1) ELISA KitMADCAM1 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1) ELISA KitMADCAM1 适用: 人 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate, Urine
Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1) ELISA KitMADCAM1 适用: 人 AA 19-317 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1) ELISA KitMADCAM1 适用: 小鼠 AA 22-365 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Cell Culture Supernatant, Serum
Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1) ELISA KitMADCAM1 适用: 人 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate, Urine
Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1) ELISA KitMADCAM1 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate, Urine
Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1) ELISA KitMADCAM1 适用: 人 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate, Urine
Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1) ELISA KitMADCAM1 适用: 大鼠 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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应用备注
- Suggested Plasma Dilution: 2 fold
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
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有效期
- 6 months
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- MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1))
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别名
- MAdCAM-1
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背景
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Gene Symbols: MADCAM1
Protein Name (Synonyms): Mucosal addressin cell adhesion molecule 1 (MAdCAM-1) ( mMAdCAM-1)
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基因ID
- 17123
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UniProt
- Q61826
抗原 See all MADCAM1 ELISA试剂盒
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