RAGE ELISA 试剂盒
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Quick Overview for RAGE ELISA 试剂盒 (ABIN625349)
抗原
See all RAGE (AGER) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 3 pg/mL
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原理
- Human RAGE ELISA Kit for cell culture supernatants, plasma, and serum samples.
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Analytical Method
- Quantitative
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特异性
- This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
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灵敏度
- 3 pg/mL
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产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Advanced Glycosylation End Product-Specific Receptor (AGER) ELISA Kit
AGER 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Advanced Glycosylation End Product-Specific Receptor (AGER) ELISA KitAGER 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Advanced Glycosylation End Product-Specific Receptor (AGER) ELISA KitAGER 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Advanced Glycosylation End Product-Specific Receptor (AGER) ELISA KitAGER 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Advanced Glycosylation End Product-Specific Receptor (AGER) ELISA KitAGER 适用: 人 AA 24-344 Colorimetric Sandwich ELISA 78-5000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Advanced Glycosylation End Product-Specific Receptor (AGER) ELISA KitAGER 适用: 小鼠 AA 24-342 Colorimetric Sandwich ELISA 78-5000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Advanced Glycosylation End Product-Specific Receptor (AGER) ELISA KitAGER 适用: 大鼠 AA 24-342 Colorimetric Sandwich ELISA 78-5000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Advanced Glycosylation End Product-Specific Receptor (AGER) ELISA KitAGER 适用: Pig Colorimetric Competition ELISA Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Advanced Glycosylation End Product-Specific Receptor (AGER) ELISA KitAGER 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Advanced Glycosylation End Product-Specific Receptor (AGER) ELISA KitAGER 适用: 人 Colorimetric Sandwich ELISA 63-4000 pg/mL Cell Lysate, Plasma, Serum
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应用备注
- Recommended Dilution for serum and plasma samples2 - 20 fold
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
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有效期
- 6 months
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: "Glycated serum albumin stimulates expression of endothelial cell specific molecule-1 in human umbilical vein endothelial cells: Implication in diabetes mediated endothelial dysfunction." in: Diabetes & vascular disease research, Vol. 12, Issue 4, pp. 290-7, (2015) (PubMed).
: "Effect of garlic component s-allyl cysteine sulfoxide on glycated human serum albumin induced activation of endothelial cells: an in vitro study." in: European review for medical and pharmacological sciences, Vol. 19, Issue 11, pp. 2125-31, (2015) (PubMed).
: "Interaction of glycated protein and DFO mimicked hypoxia in cellular responses of HUVECs." in: Molecular bioSystems, Vol. 8, Issue 10, pp. 2657-63, (2012) (PubMed).
: "Interaction of glycated human serum albumin with endothelial cells in a hemodynamic environment: structural and functional correlates." in: Molecular bioSystems, Vol. 7, Issue 11, pp. 3036-41, (2012) (PubMed).
: "C-peptide, a novel inhibitor of lung inflammation following hemorrhagic shock." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 300, Issue 5, pp. L730-9, (2011) (PubMed).
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: "Glycated serum albumin stimulates expression of endothelial cell specific molecule-1 in human umbilical vein endothelial cells: Implication in diabetes mediated endothelial dysfunction." in: Diabetes & vascular disease research, Vol. 12, Issue 4, pp. 290-7, (2015) (PubMed).
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- RAGE (AGER) (Advanced Glycosylation End Product-Specific Receptor (AGER))
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别名
- RAGE
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背景
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Gene Names: MOK RAGE RAGE1
Protein names: MAPK/MAK/MRK overlapping kinase (EC 2.7.11.22) (MOK protein kinase) (Renal tumor antigen 1) (RAGE-1)
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基因ID
- 5891
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UniProt
- Q9UQ07
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途径
- Carbohydrate Homeostasis, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, S100 Proteins
抗原 See all RAGE (AGER) ELISA试剂盒
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