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CD31 ELISA 试剂盒
PECAM1
适用: 人
Colorimetric
Sandwich ELISA
15-6000 pg/mL
Cell Culture Supernatant, Plasma, Serum
CD31ELISA Kit详情
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CD31 (PECAM1)
(Platelet/endothelial Cell Adhesion Molecule (PECAM1))
适用
All reactivities for CD31 ELISA试剂盒
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
15-6000 pg/mL
最低检测浓度
15 pg/mL
应用范围
ELISA
原理
Human PECAM-1 (CD31) ELISA Kit for cell culture supernatants, plasma, and serum samples.
样品类型
Plasma, Cell Culture Supernatant, Serum
Analytical Method
Quantitative
特异性
This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
灵敏度
15 pg/mL
产品特性
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
组件
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
试剂未包括
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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使用细节
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应用备注
Recommended Dilution for serum and plasma samples10 - 100 fold
样本量
100 μL
板类型
Pre-coated
实验流程
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
限制
仅限研究用
贮存及处理
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储存条件
-20 °C
储存方法
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
有效期
6 months
CD31目标详情
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抗原
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CD31 (PECAM1)
(Platelet/endothelial Cell Adhesion Molecule (PECAM1))
别名
PECAM-1 (PECAM1 产品 )
别名
CD31 ELISA Kit, CD31/EndoCAM ELISA Kit, GPIIA' ELISA Kit, PECA1 ELISA Kit, PECAM-1 ELISA Kit, endoCAM ELISA Kit, C85791 ELISA Kit, Cd31 ELISA Kit, Pecam ELISA Kit, platelet/endothelial cell adhesion molecule 1 ELISA Kit, platelet and endothelial cell adhesion molecule 1 ELISA Kit, pecam1 ELISA Kit, PECAM1 ELISA Kit, Pecam1 ELISA Kit
背景
Gene Names: PECAM1 Protein names: Platelet endothelial cell adhesion molecule (PECAM-1) (EndoCAM) (GPIIA') (PECA1) (CD antigen CD31)
基因ID
5175
UniProt
P16284
途径
Regulation of Actin Filament Polymerization
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