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CCL20 ELISA 试剂盒

CCL20 适用: 人 Colorimetric Sandwich ELISA 1.5-600 pg/mL Cell Lysate, Tissue Lysate
产品编号 ABIN625050
发货至: 中国
  • 抗原 See all CCL20 ELISA试剂盒
    CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))
    适用
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    1.5-600 pg/mL
    最低检测浓度
    1.5 pg/mL
    应用范围
    ELISA
    原理
    Human MIP-3 alpha (CCL20) ELISA Kit for cell and tissue lysate samples.
    样品类型
    Tissue Lysate, Cell Lysate
    Analytical Method
    Quantitative
    特异性
    The antibody pair provided in this kit recognizes human MIP-3 alpha.
    灵敏度
    1.5 pg/mL
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    • Cell lysate buffer
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    Discover our top product CCL20 ELISA Kit
  • 样本量
    100 μL
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    有效期
    6 months
  • 抗原 See all CCL20 ELISA试剂盒
    CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))
    别名
    MIP-3 alpha (CCL20 产品)
    别名
    MIP-3ALPHA ELISA Kit, CCL20 ELISA Kit, si:dkey-150o13.1 ELISA Kit, CKb4 ELISA Kit, LARC ELISA Kit, MIP-3-alpha ELISA Kit, MIP-3a ELISA Kit, MIP3A ELISA Kit, SCYA20 ELISA Kit, ST38 ELISA Kit, MIP-3A ELISA Kit, MIP-3[a] ELISA Kit, Scya20 ELISA Kit, exodus-1 ELISA Kit, C-C motif chemokine ligand 20 ELISA Kit, chemokine (C-C motif) ligand 20b ELISA Kit, chemokine (C-C motif) ligand 20 ELISA Kit, CCL20 ELISA Kit, ccl20b ELISA Kit, Ccl20 ELISA Kit
    背景
    Gene Names: CCL20 LARC MIP3A SCYA20
    Protein names: C-C motif chemokine 20 (Beta-chemokine exodus-1) (CC chemokine LARC) (Liver and activation-regulated chemokine) (Macrophage inflammatory protein 3 alpha) (MIP-3-alpha) (Small-inducible cytokine A20) [Cleaved into: CCL20(1-67), CCL20(1-64), CCL20(2-70)]
    基因ID
    6364
    UniProt
    P78556
    途径
    The Global Phosphorylation Landscape of SARS-CoV-2 Infection
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