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Angiopoietin 1 ELISA 试剂盒

ANGPT1 适用: 人 Colorimetric Sandwich ELISA 30-18000 pg/mL Cell Culture Supernatant, Plasma, Serum
产品编号 ABIN624939
发货至: 中国
  • 抗原 See all Angiopoietin 1 (ANGPT1) ELISA试剂盒
    Angiopoietin 1 (ANGPT1)
    适用
    • 11
    • 6
    • 5
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    30-18000 pg/mL
    最低检测浓度
    30 pg/mL
    应用范围
    ELISA
    原理
    Human Angiopoietin-1 ELISA Kit for cell culture supernatants, plasma, and serum samples.
    样品类型
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    特异性
    This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, Angiopoietin 2, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
    灵敏度
    < 30 pg/mL
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • 应用备注
    Recommended Dilution for serum and plasma samples3 - 30 fold
    样本量
    100 μL
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    试剂准备
    1. Bring all reagents and samples to room temperature (18 - 25 °C) before use.
      2. Sample dilution: If your samples need to be diluted, Assay Diluent A (Item D) should be used for dilution of serum/plasma samples. 1x Assay Diluent B (Item E) should be used for dilution of culture supernatants and urine. Suggested dilution for normal serum/plasma: 3-30 fold. Please note that levels of the target protein may vary between different specimens. Optimal dilution factors for each sample must be determined by the investigator.
      3. Assay Diluent B should be diluted 5-fold with deionized or distilled water.
      4. Preparation of standard: Briefly spin the vial of Item C and then add 400 µL Assay Diluent A (for serum/plasma samples) or 1x Assay Diluent B (Assay Diluent B should be diluted 5-fold with deionized or distilled water, for cell culture medium and urine) into Item C vial to prepare a 50 ng/mL standard. Dissolve the powder thoroughly by a gentle mix. Add 200 µL Angiopoietin-1 standard from the vial of Item C, into a tube with 355.6 µL Assay Diluent A or 1x Assay Diluent B to prepare a 18,000 pg/mL stock standard solution. Pipette 400 µL Assay Diluent A or 1x Assay Diluent B into each tube. Use the stock standard solution to produce a dilution series . Mix each tube thoroughly before the next transfer. Assay Diluent A or 1x Assay Diluent B serves as the zero standard (0 pg/mL). 200 µL 200 µL standard + 355.6 µL 200myl 200 µL 200 µL 200 µL 18,000 6,000 2,000 666.7 222.2 74.07 0 pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL
      5. If the Wash Concentrate (20x) (Item B) contains visible crystals, warm to room temperature and mix gently until dissolved. Dilute 20 ml of Wash Buffer Concentrate into deionized or distilled water to yield 400 ml of 1x Wash Buffer.
      6. Briefly spin the Detection Antibody vial (Item F) before use. Add 100 µL of 1x Assay Diluent B into the vial to prepare a detection antibody concentrate. Pipette up and down to mix gently (the concentrate can be stored at 4 °C for 5 days). The detection antibody concentrate should be diluted 80-fold with 1x Assay Diluent B and used in step 4 of Part VI Assay Procedure.
      7. Briefly spin the HRP-Streptavidin concentrate vial (Item G) and pipette up and down to mix gently before use. HRP-Streptavidin concentrate should be diluted 400-fold with 1x Assay Diluent B. For example: Briefly spin the vial (Item G) and pipette up and down to mix gently . Add 30 µL of HRP-Streptavidin concentrate into a tube with 12 ml 1x Assay Diluent B to prepare a 400-fold diluted HRP-Streptavidin solution (don't store the diluted solution for next day use). Mix well.
    实验流程
    1. Bring all reagents and samples to room temperature (18 - 25 °C) before use. It is recommended that all standards and samples be run at least in duplicate.
      2. Add 100 µL of each standard (see Reagent Preparation step 2) and sample into appropriate wells. Cover well and incubate for 2.5 hours at room temperature or over night at 4 °C with gentle shaking.
      3. Discard the solution and wash 4 times with 1x Wash Solution. Wash by filling each well with Wash Buffer (300 myl) using a multi-channel Pipette or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Buffer by aspirating or decanting. Invert the plate and blot it against clean paper towels.
      4. Add 100 µL of 1x prepared biotinylated antibody (Reagent Preparation step 6) to each well. Incubate for 1 hour at room temperature with gentle shaking.
      5. Discard the solution. Repeat the wash as in step
      6. Add 100 µL of prepared Streptavidin solution (see Reagent Preparation step 7) to each well. Incubate for 45 minutes at room temperature with gentle shaking.
      7. Discard the solution. Repeat the wash as in step
      8. Add 100 µL of TMB One-Step Substrate Reagent (Item H) to each well. Incubate for 30 minutes at room temperature in the dark with gentle shaking.
      9. Add 50 µL of Stop Solution (Item I) to each well. Read at 450 nm immediately.
    结果分析

    Calculate the mean absorbance for each set of duplicate standards, controls and samples, and subtract the average zero standard optical density. Plot the standard curve on log-log graph paper or using Sigma plot software, with standard concentration on the x-axis and absorbance on the y-axis. Draw the best-fit straight line through the standard points.
    Typical Data: These standard curves are for demonstration only. A standard curve must be run with each assay. Assay Diluent A Angiopoietin-1 concentration (pg/mL) O D =4 50 n m 0.01 0.1 1 10 10 100 1,000 10,000 100,000 Assay Diluent B Angiopoietin-1 concentration (pg/mL) O D =4 50 n m 0.01 0.1 1 10 10 100 1,000 10,000 100,000
    Sensitivity: The minimum detectable dose of Angiopoietin-1 is typically less than 30 pg/mL.
    Recovery: Recovery was determined by spiking various levels of human Angiopoietin-1 into human serum, plasma and cell culture media. Mean recoveries are as follows: Sample Type Average % Recovery Range ( %) Serum 94.47 81-103 Plasma 93.93 80-102 Cell culture media 95.25 83-105
    Linearity: Sample Type Serum Plasma Cell Culture Media 1:2 Average % of Expected 93 94 95 Range ( %) 80-103 82-104 84-104 1:4 Average % of Expected 96 95 97 Range ( %) 82-104 83-104 85-105
    Reproducibility: Intra-Assay: CV<10 % Inter-Assay: CV<12 %

    实验精密度
    Intra-Assay: CV< 10 % Inter-Assay: CV< 12 %
    限制
    仅限研究用
  • 注意事项
    Avoid repeated freeze-thaw cycles.
    储存条件
    -20 °C
    储存方法
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    有效期
    6 months
  • Tasaki, Shimizu, Inoue, Mizuta, Nakagishi, Wada, Yachie: "Disruption of vascular endothelial homeostasis in systemic juvenile idiopathic arthritis-associated macrophage activation syndrome: The dynamic roles of angiopoietin-1 and -2." in: Cytokine, Vol. 80, pp. 1-6, (2016) (PubMed).

    Swamynathan, Venugopal, Kannan, Thej, Kolkundar, Bhagwat, Ta, Majumdar, Balasubramanian: "Are serum-free and xeno-free culture conditions ideal for large scale clinical grade expansion of Wharton's jelly derived mesenchymal stem cells? A comparative study." in: Stem cell research & therapy, Vol. 5, Issue 4, pp. 88, (2016) (PubMed).

    Chen, Li, Zhang, Li, Wang, Guo, Luo, Wang: "Urinary angiopoietin-2 is associated with albuminuria in patients with type 2 diabetes mellitus." in: International journal of endocrinology, Vol. 2015, pp. 163120, (2015) (PubMed).

    Köksal, Ozbek, Bayraktar, Yazici: "Evaluation of angiopoietin 1 and 2, vascular endothelial growth factor, and tumor necrosis factor alpha levels in asthmatic children." in: Allergy and asthma proceedings : the official journal of regional and state allergy societies, Vol. 35, Issue 6, pp. 482-8, (2015) (PubMed).

    Fernández-Yunquera, Ripoll, Bañares, Puerto, Rincón, Yepes, Catalina, Salcedo: "Everolimus immunosuppression reduces the serum expression of fibrosis markers in liver transplant recipients." in: World journal of transplantation, Vol. 4, Issue 2, pp. 133-40, (2014) (PubMed).

    Ferrer, Wobus, List, Wehner, Schönefeldt, Brocard, Mohr, Rauner, Schmitz, Stiehler, Ehninger, Hofbauer, Bornhäuser, Platzbecker: "Mesenchymal stromal cells from patients with myelodyplastic syndrome display distinct functional alterations that are modulated by lenalidomide." in: Haematologica, Vol. 98, Issue 11, pp. 1677-85, (2014) (PubMed).

    Bhaskar, Gupta, Vishnubhatla, Kumar, Sharma, Sharma, Das, Thakur: "Angiopoietins as biomarker of disease activity and response to therapy in multiple myeloma." in: Leukemia & lymphoma, Vol. 54, Issue 7, pp. 1473-8, (2014) (PubMed).

    Vizio, Biasi, Scirelli, Novarino, Prati, Ciuffreda, Montrucchio, Poli, Bellone: "Pancreatic-carcinoma-cell-derived pro-angiogenic factors can induce endothelial-cell differentiation of a subset of circulating CD34+ progenitors." in: Journal of translational medicine, Vol. 11, pp. 314, (2013) (PubMed).

    Khan, Sharma, Kumar, Husain, Sharma: "Interrelationship and expression profiling of cyclooxygenase and angiogenic factors in Indian patients with multiple myeloma." in: Annals of hematology, Vol. 92, Issue 1, pp. 101-9, (2013) (PubMed).

    Bhaskar, Gupta, Sreenivas, Rani, Kumar, Sharma, Sharma, Sharma, Thakur: "Synergistic effect of vascular endothelial growth factor and angiopoietin-2 on progression free survival in multiple myeloma." in: Leukemia research, Vol. 37, Issue 4, pp. 410-5, (2013) (PubMed).

    Palumbo, Li: "Osteoprotegerin enhances osteogenesis of human mesenchymal stem cells." in: Tissue engineering. Part A, Vol. 19, Issue 19-20, pp. 2176-87, (2013) (PubMed).

    Gok, Erdem, Gogus, Yilmaz, Karadag, Simsek, Sagkan, Saglam, Musabak, Dinc, Pay: "Relationship of ultrasonographic findings with synovial angiogenesis modulators in different forms of knee arthritides." in: Rheumatology international, Vol. 33, Issue 4, pp. 879-85, (2013) (PubMed).

    Nazarov, Lee, Soupene, Etemad, Knapik, Green, Bashkirova, Fang, Matthay, Kuypers, Serikov: "Multipotent stromal stem cells from human placenta demonstrate high therapeutic potential." in: Stem cells translational medicine, Vol. 1, Issue 5, pp. 359-72, (2012) (PubMed).

    Karapınar, Esen, Emiroğlu, Akçakoyun, Pala, Kargın, Izgi, Kirma, Esen: "Serum levels of angiopoietin-1 in patients with pulmonary hypertension due to mitral stenosis." in: Heart and vessels, Vol. 26, Issue 5, pp. 536-41, (2012) (PubMed).

    Liu, Zeng, Zhang: "Osteogenic and angiogenic effects of mesenchymal stromal cells with co-transfected human Ang-1 gene and BMP2 gene." in: Biotechnology letters, Vol. 33, Issue 10, pp. 1933-8, (2012) (PubMed).

    Reed, Masoumi, Elhassan, McFann, Cadnapaphornchai, Maahs, Snell-Bergeon, Schrier: "Angiogenic growth factors correlate with disease severity in young patients with autosomal dominant polycystic kidney disease." in: Kidney international, Vol. 79, Issue 1, pp. 128-34, (2010) (PubMed).

  • 抗原 See all Angiopoietin 1 (ANGPT1) ELISA试剂盒
    Angiopoietin 1 (ANGPT1)
    别名
    Angiopoietin-1 (ANGPT1 产品)
    别名
    AGP1 ELISA Kit, AGPT ELISA Kit, ANG1 ELISA Kit, 1110046O21Rik ELISA Kit, Ang-1 ELISA Kit, Ang1 ELISA Kit, Agpt ELISA Kit, Agpt1 ELISA Kit, ang1 ELISA Kit, wu:fc76g06 ELISA Kit, ANG-1 ELISA Kit, angpt1 ELISA Kit, MGC80788 ELISA Kit, angiopoietin 1 ELISA Kit, angiopoietin 1 S homeolog ELISA Kit, ANGPT1 ELISA Kit, Angpt1 ELISA Kit, angpt1 ELISA Kit, angpt1.S ELISA Kit
    背景
    The Human Angiopoietin-1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Angiopoietin-1 in serum, plasma, cell culture supernatants and urine. This assay employs an antibody specific for human Angiopoietin-1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Angiopoietin-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Angiopoietin-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Angiopoietin-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Reproducibility: Intra-Assay: CV<10% Inter-Assay: CV<12%.
    基因ID
    284
    UniProt
    Q15389
    途径
    RTK signaling, Glycosaminoglycan Metabolic Process
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