电话:
+86 (0512) 65829739
传真:
+86 (010) 6788 5057
电子邮件:
orders@antibodies-online.cn

Estrogen Receptor alpha ELISA 试剂盒

This anti-Estrogen Receptor alpha antibody (ABIN6244372) is a antibody detecting Estrogen Receptor alpha in ELISA. Suitable for Human.
产品编号 ABIN6244372
发货至: 中国

Quick Overview for Estrogen Receptor alpha ELISA 试剂盒 (ABIN6244372)

抗原

See all Estrogen Receptor alpha (ESR1) ELISA试剂盒
Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

抗原表位

pSer118

适用

  • 15
  • 9
  • 6
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1

检测方法

Colorimetric

实验类型

Sandwich ELISA

应用范围

ELISA

样品类型

Cell Samples, Tissue Lysate
  • 原理

    Human Phospho-Estrogen receptor (Ser118) ELISA Kit. This assay semi-quantitatively measures Estrogen receptor phosphorylated at Serine-118 in cell lysate samples.

    Analytical Method

    Semi-Quantitative

    特异性

    This ELISA kit recognizes Human Estrogen receptor phosphorylated at site Serine-118.

    产品特性

    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • HRP-Conjugated Secondary Antibody
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample

    组件

    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • HRP-Conjugated Secondary Antibody
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample

    试剂未包括

    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
  • 应用备注

    Optimal working dilution should be determined by the investigator.

    板类型

    Pre-coated

    实验流程

    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.

    限制

    仅限研究用
  • 储存条件

    -20 °C

    储存方法

    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

    有效期

    6 months
  • 抗原 See all Estrogen Receptor alpha (ESR1) ELISA试剂盒

    Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

    别名

    Estrogen receptor

    基因ID

    2099

    UniProt

    P03372

    途径

    Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly
You are here:
Chat with us!