Estrogen Receptor alpha ELISA 试剂盒
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- 抗原 See all Estrogen Receptor alpha (ESR1) ELISA试剂盒
- Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- DNA-Binding ELISA
- 应用范围
- ELISA
- 原理
- DNA-binding ELISA that facilitate the study of transcription factor activation in mammalien tissue and cell culture extracts.
- 品牌
- TransAM®
- 样品类型
- Cell Extracts, Tissue Samples
- Analytical Method
- Quantitative
- 特异性
- TransAM ER Kits are tested for sensitivity in detecting ER in nuclear extracts from MCF-7 cells that are either untreated or post-treated with H2O2.
- 产品特性
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Transcription factors are DNA-binding proteins that tightly regulate gene expression. They consist of two distinct domains - one that displays high affinity for a specific DNA sequence and one that confers transcriptional activity. Transcription factors are activated by phosphorylation of specific residues or by processing bound inhibitory proteins. Understanding and quantifying transcription factors is essential for the study of cell functions in relation to differentiation, brain activity, immune response, inflammation and various disease states. TransAM® Kits are sensitive, non-radioactive transcription factor ELISA kits that facilitate the study of transcription factor activation in mammalian tissue and cell extracts.
TransAM® Kits are DNA-binding ELISAs that facilitate the study of transcription factor activation in mammalian tissue and cell extracts. Each kit includes a 96-stripwell plate in which multiple copies of a specific double-stranded oligonucleotide have been immobilized. When nuclear or whole-cell extract is added, activated transcription factor of interest binds the oligonucleotide at its consensus binding site and is quantified using the included antibody, which is specific for the bound, active form of the transcription factor being studied. - 组件
- One or five 96-well plate(s) with plate sealer(s), primary antibody, HRP-conjugated secondary antibody, wild-type and mutated competitor oligonucleotides, positive control cell extract, DTT, Protease Inhibitor Cocktail, Lysis, Binding, 10X Washing and 10X Antibody Binding Buffers, and Developing and Stop Solutions.
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Estrogen Receptor 1 (ESR1) ELISA Kit
ESR1 适用: 人 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Estrogen Receptor 1 (ESR1) ELISA KitESR1 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Estrogen Receptor 1 (ESR1) ELISA KitESR1 适用: 人 Colorimetric Sandwich ELISA 3.12 pg/mL - 200 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Estrogen Receptor 1 (ESR1) ELISA KitESR1 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Estrogen Receptor 1 (ESR1) ELISA KitESR1 适用: 人 pSer118 Colorimetric Sandwich ELISA Cell Samples, Tissue Lysate
Estrogen Receptor 1 (ESR1) ELISA KitESR1 适用: 人 pSer118 Colorimetric Sandwich ELISA Cell Samples, Tissue Lysate
Estrogen Receptor 1 (ESR1) ELISA KitESR1 适用: 小鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Estrogen Receptor 1 (ESR1) ELISA KitESR1 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma, Serum
Estrogen Receptor 1 (ESR1) ELISA KitESR1 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Estrogen Receptor 1 (ESR1) ELISA KitESR1 适用: 人 Colorimetric DNA-Binding ELISA Cell Lysate, Nuclear Extract
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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These extracts are diluted down to 0.313 μg/well and assayed using the TransAM ER Kit. The ratio of the signals from the treated over the untreated cells must be above 4. Lot No. 01403001 was developed for 5 minutes. It gave a ratio of 17 (Figure 1). The basal level of ER expression, and this ratio, may vary depending on the cell type tested and the treatment used. TransAM ER Kits are also tested for specificity in detecting ER activation. TransAM ER assays are performed in the presence of an excess of oligonucleotide containing a wild-type or mutated ER consensus binding site (Figure 2). At 20X excess, the wild-type oligonucleotide prevents binding to the probe immobilized on the plate. Conversely, the mutated oligonucleotide has little effect on ER binding.
- 实验时间
- 5 h
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- 4 °C/-20 °C/-80 °C
- 储存方法
- Except for the cell extract that must be kept at -80°C, the kit components can be stored at -20°C prior to first use. Then, we recommend storing the kit at 4°C except for the primary antibody, oligonucleotides, DTT, Poly [d(I-C)] and Protease Inhibitor Cocktail that should be kept at -20°C, and the cell extract at -80°C. This product is guaranteed for 6 months from date of receipt.
- 有效期
- 6 months
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- 抗原 See all Estrogen Receptor alpha (ESR1) ELISA试剂盒
- Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))
- 别名
- Estrogen Receptor 1 (ESR1 产品)
- 别名
- LOC398734 ELISA Kit, AA420328 ELISA Kit, AU041214 ELISA Kit, ER-alpha ELISA Kit, ER[a] ELISA Kit, ERa ELISA Kit, ERalpha ELISA Kit, ESR ELISA Kit, Estr ELISA Kit, Estra ELISA Kit, Nr3a1 ELISA Kit, NR3A1 ELISA Kit, eralpha ELISA Kit, zfER[a] ELISA Kit, ER ELISA Kit, ESRA ELISA Kit, ESTRR ELISA Kit, Era ELISA Kit, akap12 ELISA Kit, cb27 ELISA Kit, esr1 ELISA Kit, fb72g12 ELISA Kit, id:ibd1202 ELISA Kit, sb:cb27 ELISA Kit, si:ch73-192g21.1 ELISA Kit, wu:fb72g12 ELISA Kit, wu:fc21c03 ELISA Kit, Esr ELISA Kit, RNESTROR ELISA Kit, ERALPHA ELISA Kit, er ELISA Kit, esr ELISA Kit, nr3a1 ELISA Kit, XER ELISA Kit, era ELISA Kit, esra ELISA Kit, ERbeta ELISA Kit, xesr-1 ELISA Kit, xlERalpha1 ELISA Kit, xlERalpha2 ELISA Kit, ESR1 ELISA Kit, estrogen receptor 1 L homeolog ELISA Kit, estrogen receptor 1 (alpha) ELISA Kit, estrogen receptor 1 ELISA Kit, A kinase (PRKA) anchor protein 12b ELISA Kit, esr1.L ELISA Kit, Esr1 ELISA Kit, ESR1 ELISA Kit, esr1 ELISA Kit, akap12b ELISA Kit
- 途径
- Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly
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