Growth Hormone 1 ELISA 试剂盒
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Quick Overview for Growth Hormone 1 ELISA 试剂盒 (ABIN577093)
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See all Growth Hormone 1 (GH1) ELISA试剂盒适用
检测方法
实验类型
应用范围
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原理
- This hGH enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to hGH. Standards or samples are then added to the microtiter plate wells and incubated. After wash all wells, hGH if present, will bind to the antibody pre-coated on the wells. In order to quantitatively determine the amount of hGH present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated monoclonal antibody specific to hGH is added to each well to “sandwich” the hGH immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, a TMB (3,3', 5,5' Tetramethyl-benzidene) substrate solution is added to each well. This enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain hGH and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.
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Analytical Method
- Quantitative
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灵敏度
- The minimal detectable concentration of hGH by this assay is estimated to be 0.1 ng/mL.
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组件
- Standards: 1 set/2 vials
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Growth Hormone 1 (GH1) ELISA Kit
GH1 适用: 小鸡 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum
Growth Hormone 1 (GH1) ELISA KitGH1 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Growth Hormone 1 (GH1) ELISA KitGH1 适用: Pig Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
Growth Hormone 1 (GH1) ELISA KitGH1 适用: 犬 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum
Growth Hormone 1 (GH1) ELISA KitGH1 适用: Cow Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Growth Hormone 1 (GH1) ELISA KitGH1 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Growth Hormone 1 (GH1) ELISA KitGH1 适用: 犬 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Growth Hormone 1 (GH1) ELISA KitGH1 适用: 小鸡 Colorimetric Competition ELISA 123.5 pg/mL - 10000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Growth Hormone 1 (GH1) ELISA KitGH1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Growth Hormone 1 (GH1) ELISA KitGH1 适用: 犬 Colorimetric Competition ELISA 123.5 pg/mL - 10000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存液
- Without preservative
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- Growth Hormone 1 (GH1)
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物质类
- Hormone
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背景
- Human growth hormone (GH) is a 22kDa monomeric protein produced and stored in somatotrophs in the anterior pituitary gland. GH is released from the pituitary into the bloodstream in a pulsatile manner under the regulatory control of hypothalamic somatostatin (SS) and GH-releasing factor (GHRF) [1]. The timing and frequency of GH release appears to be regulated by somatostatin, while the amplitude of GH release is determined by GHRF. A minor fraction (~10%) of GH in circulation exists in a smaller 20 kDa form [2]. GH has profound effects on tissue growth and metabolism, which is thought to be mediated through GH-dependent production of IGF-I and IGF-II, and their associated binding proteins. GH apparently stimulates IGF production after binding to specific cell surface receptors in the liver and, possibly, other tissues. Almost 50% of GH in circulation is bound to a high affinity GH binding protein (GHBP), which represents the extracellular domain of the GH receptor. Deficient GH secretion can occur in a number of clinical conditions [3]. However, evaluation of GH deficiency is complicated by the episodic nature of GH secretion and low circulating levels. A variety of physiologic and pharmacologic stimuli have been used to stimulate pituitary GH release during testing and failure to achieve a normal serum GH level in response to at least 2 GH stimulation or provocative tests is considered to be a diagnostic of GH deficiency [4]. The definition of a normal serum GH response is controversial, although published values generally range from 5 to 10 ng/mL. GH excess (or acromegaly) can be caused either by direct GH hypersecretion or GH excess secondary to GHRF hypersecretion.
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途径
- NF-kappaB Signaling, JAK/STAT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Response to Growth Hormone Stimulus, Regulation of Hormone Biosynthetic Process
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