C3 ELISA 试剂盒
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Quick Overview for C3 ELISA 试剂盒 (ABIN5706900)
抗原
See all C3 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 1.56 ng/mL
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Analytical Method
- Quantitative
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灵敏度
- 0.64 ng/mL
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Complement Component 3 (C3) ELISA Kit
C3 适用: 人 Colorimetric Sandwich ELISA 7.8 ng/mL - 500 ng/mL Plasma, Serum
Complement Component 3 (C3) ELISA KitC3 适用: 小鼠 Colorimetric Sandwich ELISA 3.13 ng/mL - 200 ng/mL Cell Culture Supernatant, Plasma, Serum
Complement Component 3 (C3) ELISA KitC3 适用: Pig Colorimetric Competition ELISA 0.625 μg/mL - 10 μg/mL Plasma, Serum, Tissue Homogenate
Complement Component 3 (C3) ELISA KitC3 适用: 小鼠 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Plasma, Serum
Complement Component 3 (C3) ELISA KitC3 适用: 大鼠 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Complement Component 3 (C3) ELISA KitC3 适用: Pig Colorimetric Sandwich ELISA 62.5 ng/mL - 4000 ng/mL Plasma, Serum
Complement Component 3 (C3) ELISA KitC3 适用: Cow Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Plasma, Serum
Complement Component 3 (C3) ELISA KitC3 适用: Pig Colorimetric Sandwich ELISA 0.234 ng/mL - 15 ng/mL Plasma, Serum, Tissue Homogenate
Complement Component 3 (C3) ELISA KitC3 适用: 人 Colorimetric Sandwich ELISA 7.813 ng/mL - 500 ng/mL Plasma, Serum, Tissue Homogenate
Complement Component 3 (C3) ELISA KitC3 适用: 小鼠 Colorimetric Sandwich ELISA 3.125 ng/mL - 200 ng/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Pre-coated, ready to use 96-well strip plate,
Standard (freeze dried),
Standard Diluent,
Detection Reagent A,
Detection Reagent B,
Assay Diluent A,
Assay Diluent B,
TMB substrate,
Stop Solution,
Wash Buffer(30 x concenthumane),
Plate sealer for 96 wells,
Instruction manual -
样本量
- 100 µL
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实验时间
- 1 - 4.5 h
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板类型
- Pre-coated
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实验流程
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1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8. Add 50µL Stop Solution. Read at 450nm immediately. -
限制
- 仅限研究用
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储存条件
- 4 °C
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有效期
- 12 months
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- C3 (Complement Component 3 (C3))
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背景
- Alternative name: ASP, CPAMD1, Complement Protein C3, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, C3a anaphylatoxin, Acylation stimulating protein
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途径
- Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling
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