DKK4 ELISA 试剂盒
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Quick Overview for DKK4 ELISA 试剂盒 (ABIN5692231)
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适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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原理
- Sandwich Human DKK-4 / Dickkopf 4 ELISA Kit to quantitate Human DKK4 in cell culture supernatants, serum and plasma (heparin, EDTA).
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: L19-L224Capture antibody and Detection antibody: a monoclonal antibody from mouse, a biotinylated polyclonal antibody from goat
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Dickkopf Homolog 4 (Xenopus Laevis) (DKK4) ELISA Kit
DKK4 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Dickkopf Homolog 4 (Xenopus Laevis) (DKK4) ELISA KitDKK4 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum
Dickkopf Homolog 4 (Xenopus Laevis) (DKK4) ELISA KitDKK4 适用: 小鼠 AA 19-221 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
Dickkopf Homolog 4 (Xenopus Laevis) (DKK4) ELISA KitDKK4 适用: 人 Colorimetric Sandwich ELISA 10-10000 pg/mL Cell Culture Supernatant, Plasma, Serum
Dickkopf Homolog 4 (Xenopus Laevis) (DKK4) ELISA KitDKK4 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum
Dickkopf Homolog 4 (Xenopus Laevis) (DKK4) ELISA KitDKK4 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum
Dickkopf Homolog 4 (Xenopus Laevis) (DKK4) ELISA KitDKK4 适用: 小鼠 Colorimetric Sandwich ELISA 78-5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Dickkopf Homolog 4 (Xenopus Laevis) (DKK4) ELISA KitDKK4 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum
Dickkopf Homolog 4 (Xenopus Laevis) (DKK4) ELISA KitDKK4 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 5.6%, 5.3%, 7.6%
Inter-Assay Precision (CV%): 7.8%, 5.6%, 8.9% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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- DKK4 (Dickkopf Homolog 4 (Xenopus Laevis) (DKK4))
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别名
- DKK4
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背景
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Background: DKK4 is a membersof the Dickkopf family which are negative regulators of the growth-promoting Wnt / beta-catenin signaling pathway. By FISH, the DKK4 gene was mapped to chromosome 8p11.2-p11.1. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. Activity of this protein is modulated by binding to the Wnt co-receptor and the co-factor kremen 2. The expression of DKK4 was significantly reduced in hepatocellular carcinomas (HCCs), particularly in HCCs that showed cytoplasmic beta- catenin accumulation. DKK4 expression was also reduced in HCC cell lines. Ectopic expression of DKK4 in 2 HCC cell lines reduced beta-catenin and cyclin D1 protein levels and attenuated beta-catenin activity in reporter gene assays.
Gene Full Name: dickkopf Wnt signaling pathway inhibitor 4
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基因ID
- 27121
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UniProt
- Q9UBT3
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途径
- WNT signaling
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