KIT Ligand ELISA 试剂盒
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Quick Overview for KIT Ligand ELISA 试剂盒 (ABIN5690995)
抗原
See all KIT Ligand (KITLG) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.15 ng/mL
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原理
- Porcine SCF (Kit ligand / MGF) ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
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Analytical Method
- Quantitative
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特异性
- This ELISA antibody pair detects porcine SCF. Other species not determined.
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产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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组件
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- Pre-Coated 96-well Strip Microplate
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- Wash Buffer
- TMB One-Step Substrate Reagent
- Stop Solution
- Assay Diluent(s)
- Adhesive Sealing Film
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试剂未包括
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- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Log-log graph paper or computer and software for ELISA data analysis
- Tubes to prepare standard or sample dilutions
- Plastic wrap
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KIT Ligand (KITLG) ELISA Kit
KITLG 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
KIT Ligand (KITLG) ELISA KitKITLG 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
KIT Ligand (KITLG) ELISA KitKITLG 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
KIT Ligand (KITLG) ELISA KitKITLG 适用: 小鼠 AA 26-190 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Serum
KIT Ligand (KITLG) ELISA KitKITLG 适用: 小鸡 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
KIT Ligand (KITLG) ELISA KitKITLG 适用: Pig Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
KIT Ligand (KITLG) ELISA KitKITLG 适用: 大鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
KIT Ligand (KITLG) ELISA KitKITLG 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
KIT Ligand (KITLG) ELISA KitKITLG 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
KIT Ligand (KITLG) ELISA KitKITLG 适用: Pig Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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应用备注
- Suggested Plasma Dilution: 2 fold
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL standard or sample to each well. Incubate 2.5 hours at room temperature.
- Add 100 μL prepared biotin antibody to each well. Incubate 1 hour at room temperature.
- Add 100 μL prepared Streptavidin solution to each well. Incubate 45 minutes at room temperature.
- Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 μL Stop Solution to each well. Read at 450 nm immediately
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
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有效期
- 6 months
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- KIT Ligand (KITLG)
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别名
- SCF (Kit ligand/MGF)
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背景
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Gene Symbols: KITLG, MGF
Protein Name (Synonyms): Kit ligand (Mast cell growth factor) (MGF) (Stem cell factor) (SCF) (c-Kit ligand) [Cleaved into: Soluble KIT ligand (sKITLG)
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基因ID
- 100626805397509, 397509
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UniProt
- Q29030
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途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
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