TSC1 ELISA 试剂盒
TSC1
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
-
- 抗原 See all TSC1 ELISA试剂盒
- TSC1 (Tuberous Sclerosis 1 (TSC1))
-
适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human TSC1/Hamartin ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Human TSC1.
- 产品特性
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
-
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product TSC1 ELISA Kit
-
-
- 板类型
- Pre-coated
- 实验流程
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
-
- 储存条件
- 4 °C
- 有效期
- 6 months
-
- 抗原 See all TSC1 ELISA试剂盒
- TSC1 (Tuberous Sclerosis 1 (TSC1))
- 别名
- TSC1 (Hamartin (TSC1 产品)
- 别名
- LAM ELISA Kit, TSC ELISA Kit, hamartin ELISA Kit, mKIAA0243 ELISA Kit, tsc1 ELISA Kit, wu:fc38b04 ELISA Kit, fa99f04 ELISA Kit, wu:fa99f04 ELISA Kit, zgc:63657 ELISA Kit, TSC complex subunit 1 ELISA Kit, tuberous sclerosis 1 ELISA Kit, TSC complex subunit 1b ELISA Kit, TSC complex subunit 1a ELISA Kit, TSC1 ELISA Kit, Tsc1 ELISA Kit, tsc1b ELISA Kit, tsc1 ELISA Kit, tsc1a ELISA Kit
- 基因ID
- 7248
- UniProt
- Q92574
- 途径
- RTK signaling, AMPK Signaling, Regulation of Cell Size, Tube Formation
-
You are here:
