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Estrogen Receptor alpha ELISA 试剂盒

This anti-Estrogen Receptor alpha antibody (ABIN5690752) is a antibody detecting Estrogen Receptor alpha in ELISA. Suitable for Human.
产品编号 ABIN5690752
发货至: 中国

Quick Overview for Estrogen Receptor alpha ELISA 试剂盒 (ABIN5690752)

抗原

See all Estrogen Receptor alpha (ESR1) ELISA试剂盒
Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

适用

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检测方法

Colorimetric

实验类型

DNA-Binding ELISA

应用范围

ELISA

样品类型

Cell Lysate, Nuclear Extract
  • 原理

    Human ER-alpha Transcription Factor Activity Assay. This assay uses a dsDNA coated plate with canonical ER-alpha binding sequences to semi-quantitatively detect active ER-alpha in lysates or nuclear extracts.

    Analytical Method

    Semi-Quantitative

    特异性

    The olionucleotide/antibody pair provided in this kit recognizes mouse ER-alpha in whole lysates and nuclear extracts.

    产品特性

    • Specific transcription factor-DNA binding assay
    • Perfect alternative to EMSA
    • Easy to perform in an ELISA format
    • Non-radioactive assay
    • High throughput (96 well plate format)
    • Assay can be completed within 5 hours

    组件

    • 96-well Strip Microplate pre-coated with DNA probes
    • DNA Binding Buffer
    • Positive Control Sample
    • Specific Competitor DNA probe
    • Non-specific Competitor DNA probe
    • Assay Reagent
    • DTT
    • Wash Buffer
    • Primary Antibody
    • HRP-conjugated Secondary Antibody
    • TMB One-Step Substrate Reagent
    • Stop Solution

    试剂未包括

    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions Absorbent paper
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
  • 板类型

    Pre-coated

    实验流程

    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared HRP-secondary antibody to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.

    限制

    仅限研究用
  • 储存条件

    4 °C

    储存方法

    Upon receipt, the positive control should be removed and stored at -20° or -80°C. The remainder of the kit can be stored for up to 6 months at 2-8°C from the date of shipment. Opened Microplate Wells or reagents may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: The kit can be used within one year if the whole kit is stored at -20°C upon receipt. Avoid repeated freeze-thaw cycles.

    有效期

    6 months
  • 抗原 See all Estrogen Receptor alpha (ESR1) ELISA试剂盒

    Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

    别名

    ER-alpha

    基因ID

    2099

    UniProt

    P03372

    途径

    Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly
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