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Estrogen Receptor alpha ELISA 试剂盒

Name: Estrogen Receptor alpha ELISA 试剂盒
SKU: ABIN5690752
Availability: OutOfStock

This 人 Estrogen Receptor alpha ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 Estrogen Receptor alpha.

Transcription factor assay of ER-alpha from nuclear extracts of MCF-7 cells or MCF-7 cells treated with H2O2 (200uM) for 3 hr with the specific competitor or non-specific competitor. (Estrogen Receptor alpha ELISA 试剂盒)

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Transcription factor assay of ER-alpha from nuclear extracts of MCF-7 cells or MCF-7 cells treated with H2O2 (200uM) for 3 hr with Activity Assay Kit. (Estrogen Receptor alpha ELISA 试剂盒)

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产品编号 ABIN5690752

发货至: 中国

Quick Overview for Estrogen Receptor alpha ELISA 试剂盒 (ABIN5690752)

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See all Estrogen Receptor alpha (ESR1) ELISA试剂盒

Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

适用

人

检测方法

Colorimetric

实验类型

DNA-Binding ELISA

应用范围

ELISA

样品类型

Cell Lysate, Nuclear Extract

Discover our top product ESR1 ELISA Kit

原理

Human ER-alpha Transcription Factor Activity Assay. This assay uses a dsDNA coated plate with canonical ER-alpha binding sequences to semi-quantitatively detect active ER-alpha in lysates or nuclear extracts.

Analytical Method

Semi-Quantitative

特异性

The olionucleotide/antibody pair provided in this kit recognizes mouse ER-alpha in whole lysates and nuclear extracts.

产品特性
- Specific transcription factor-DNA binding assay
- Perfect alternative to EMSA
- Easy to perform in an ELISA format
- Non-radioactive assay
- High throughput (96 well plate format)
- Assay can be completed within 5 hours
组件
- 96-well Strip Microplate pre-coated with DNA probes
- DNA Binding Buffer
- Positive Control Sample
- Specific Competitor DNA probe
- Non-specific Competitor DNA probe
- Assay Reagent
- DTT
- Wash Buffer
- Primary Antibody
- HRP-conjugated Secondary Antibody
- TMB One-Step Substrate Reagent
- Stop Solution
试剂未包括
- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions Absorbent paper
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
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Estrogen Receptor 1 (ESR1) ELISA Kit
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ESR1 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma, Serum

Estrogen Receptor 1 (ESR1) ELISA Kit
ESR1 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate

Estrogen Receptor 1 (ESR1) ELISA Kit
ESR1 适用: 人 Colorimetric DNA-Binding ELISA Cell Extracts, Tissue Samples

板类型

Pre-coated

实验流程
1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared HRP-secondary antibody to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.
限制

仅限研究用
储存条件

4 °C

储存方法

Upon receipt, the positive control should be removed and stored at -20° or -80°C. The remainder of the kit can be stored for up to 6 months at 2-8°C from the date of shipment. Opened Microplate Wells or reagents may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: The kit can be used within one year if the whole kit is stored at -20°C upon receipt. Avoid repeated freeze-thaw cycles.

有效期

6 months
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Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

别名

ER-alpha

基因ID

2099

UniProt

P03372

途径

Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly