RPS6KA3 ELISA 试剂盒
This 人, 小鼠 和 大鼠 RPS6KA3 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人, 小鼠 和 大鼠 RPS6KA3.
Quick Overview for RPS6KA3 ELISA 试剂盒 (ABIN5690735)
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RPS6KA3
(Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 3 (RPS6KA3))
适用
人, 小鼠, 大鼠
检测方法
Colorimetric
实验类型
Cell ELISA
应用范围
ELISA
样品类型
Adherent Cell Culture
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原理
- Human, Mouse and Rat Phospho-RSK2 (Ser386) and Total RSK2 Cell-Based ELISA Cell-Based ELISA Kit. This assay semi-quantitatively measures RSK2 phosphorylated at Serine-386 as well as total RSK2 in adherent cell lines.
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Analytical Method
- Semi-Quantitative
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特异性
- The antibodies provided in this kit recognizes Human, Mouse and Rat RSK2 phosphorylated at site Serine-386 as well as total RSK2.
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产品特性
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- Rapidly measure phosphorylated protein in adherent cell lines
- Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
- No sample lysis is needed
- Compatible with a standard ELISA plate reader
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组件
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- Uncoated 96-well Strip Microplate
- Wash Buffers
- Fixing Solution
- Quenching Buffer
- Blocking Buffer
- Anti-phospho antibody
- Anti-pan antibody
- HRP-Conjugated Secondary Antibody
- TMB One-Step Substrate
- Stop Solution
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试剂未包括
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
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板类型
- Uncoated
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实验流程
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
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有效期
- 6 months
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- RPS6KA3 (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 3 (RPS6KA3))
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别名
- RSK2
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基因ID
- 6197
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UniProt
- P51812, P18654, D3Z8E0
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途径
- MAPK Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades
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