NME1 ELISA 试剂盒
Quick Overview for NME1 ELISA 试剂盒 (ABIN5674914)
抗原
See all NME1 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
-
-
最低检测浓度
- 0.156 ng/mL
-
Analytical Method
- Quantitative
-
灵敏度
- 0053 ng/mL
-
组件
-
Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual -
试剂未包括
-
1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.
-
-
Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1) ELISA Kit
NME1 适用: 人 Colorimetric Sandwich ELISA 18.75 pg/mL - 1200 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1) ELISA KitNME1 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1) ELISA KitNME1 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
实验时间
- 1 - 4.5 h
-
板类型
- Pre-coated
-
实验流程
-
1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately. -
限制
- 仅限研究用
-
-
-
储存条件
- 4 °C
-
有效期
- 12 months
-
-
- NME1 (Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1))
-
别名
- Non Metastatic Cells 1, Protein NM23A Expressed In (NME1)
-
背景
- Alternative name: AWD, GAAD, NDPKA, NM23-H1, Nucleoside diphosphate kinase A, Granzyme A-activated DNase, Metastasis inhibition factor nm23, Tumor metastatic process-associated protein
-
基因ID
- 18102
-
UniProt
- P15532
-
途径
- Apoptosis, Nucleotide Phosphorylation, Carbohydrate Homeostasis, Ribonucleoside Biosynthetic Process
抗原 See all NME1 ELISA试剂盒
-
