PRKDC ELISA 试剂盒
PRKDC
适用: 人
Colorimetric
Sandwich ELISA
0.312 ng/mL - 20 ng/mL
Tissue Homogenate
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- 抗原 See all PRKDC ELISA试剂盒
- PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.312 ng/mL - 20 ng/mL
- 最低检测浓度
- 0.312 ng/mL
- 应用范围
- ELISA
- 样品类型
- Tissue Homogenate
- Analytical Method
- Quantitative
- 灵敏度
- 0125 ng/mL
- 组件
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Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual - 试剂未包括
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1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution. - Top Product
- Discover our top product PRKDC ELISA Kit
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Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC) ELISA Kit
PRKDC 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Saliva, Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验时间
- 1 - 4.5 h
- 板类型
- Pre-coated
- 实验流程
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1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately. - 限制
- 仅限研究用
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- 储存条件
- 4 °C
- 有效期
- 12 months
-
- 抗原 See all PRKDC ELISA试剂盒
- PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC))
- 别名
- Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC) (PRKDC 产品)
- 别名
- DNA-PKcs ELISA Kit, DNAPK ELISA Kit, DNPK1 ELISA Kit, HYRC ELISA Kit, HYRC1 ELISA Kit, XRCC7 ELISA Kit, p350 ELISA Kit, DNA-PKCS ELISA Kit, dna-pkcs ELISA Kit, dnapk ELISA Kit, dnpk1 ELISA Kit, hyrc ELISA Kit, hyrc1 ELISA Kit, prkdc-A ELISA Kit, xrcc7 ELISA Kit, PRKDC ELISA Kit, AI326420 ELISA Kit, AU019811 ELISA Kit, DNAPDcs ELISA Kit, DOXNPH ELISA Kit, dxnph ELISA Kit, p460 ELISA Kit, scid ELISA Kit, slip ELISA Kit, protein kinase, DNA-activated, catalytic polypeptide ELISA Kit, protein kinase, DNA-activated, catalytic polypeptide L homeolog ELISA Kit, DNA-dependent protein kinase catalytic subunit ELISA Kit, protein kinase, DNA activated, catalytic polypeptide ELISA Kit, PRKDC ELISA Kit, prkdc.L ELISA Kit, LOC100633457 ELISA Kit, Prkdc ELISA Kit
- 背景
- Alternative name: HYRC, HYRC1, DNPK1, p350, DNAPK, XRCC7, DNA-PKcs
- 基因ID
- 5591
- UniProt
- P78527
- 途径
- DNA Damage Repair, Production of Molecular Mediator of Immune Response
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