LC3B ELISA 试剂盒
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- 抗原 See all LC3B (MAP1LC3B) ELISA试剂盒
- LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 灵敏度
- 0068 ng/mL
- 组件
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Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual - 试剂未包括
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1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution. - Featured
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Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) ELISA Kit
MAP1LC3B 适用: 小鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) ELISA KitMAP1LC3B 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) ELISA KitMAP1LC3B 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) ELISA KitMAP1LC3B 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) ELISA KitMAP1LC3B 适用: 人 Colorimetric Sandwich ELISA 18.75-1200 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验时间
- 1 - 4.5 h
- 板类型
- Pre-coated
- 实验流程
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1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately. - 限制
- 仅限研究用
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- 储存条件
- 4 °C
- 有效期
- 12 months
-
- 抗原 See all LC3B (MAP1LC3B) ELISA试剂盒
- LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))
- 别名
- Microtubule Associated Protein 1 Light Chain 3 Beta (MAP1LC3b) (MAP1LC3B 产品)
- 别名
- ATG8F ELISA Kit, LC3B ELISA Kit, MAP1A/1BLC3 ELISA Kit, MAP1LC3B-a ELISA Kit, Map1lc3 ELISA Kit, Mpl3 ELISA Kit, zbs559 ELISA Kit, 1010001C15Rik ELISA Kit, Atg8 ELISA Kit, LC3b ELISA Kit, zgc:56434 ELISA Kit, wu:fb60g11 ELISA Kit, map1lc3b ELISA Kit, MGC76283 ELISA Kit, MAP1LC3B ELISA Kit, microtubule associated protein 1 light chain 3 beta ELISA Kit, microtubule-associated protein 1 light chain 3 beta ELISA Kit, microtubule associated protein 1 light chain 3 beta L homeolog ELISA Kit, MAP1LC3B ELISA Kit, Map1lc3b ELISA Kit, map1lc3b ELISA Kit, map1lc3b.L ELISA Kit
- 背景
- Alternative name: MAP1A/1BLC3, ATG8F, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3-like protein 2
- 基因ID
- 81631
- UniProt
- Q9GZQ8
- 途径
- Autophagy
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