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NKAP ELISA 试剂盒

NKAP 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Tissue Homogenate
产品编号 ABIN5674703
发货至: 中国
  • 抗原 See all NKAP ELISA试剂盒
    NKAP (NFKB Activating Protein (NKAP))
    适用
    • 2
    • 2
    • 1
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    31.25 pg/mL - 2000 pg/mL
    最低检测浓度
    31.25 pg/mL
    应用范围
    ELISA
    样品类型
    Tissue Homogenate
    Analytical Method
    Quantitative
    灵敏度
    13.8 pg/mL
    组件
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    试剂未包括
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • 应用备注
    Optimal working dilution should be determined by the investigator.
    实验时间
    1 - 4.5 h
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    限制
    仅限研究用
  • 储存条件
    4 °C
    有效期
    12 months
  • 抗原 See all NKAP ELISA试剂盒
    NKAP (NFKB Activating Protein (NKAP))
    Abstract
    NKAP 产品
    别名
    DSMA4 ELISA Kit, GEF720 ELISA Kit, Syx ELISA Kit, Tech ELISA Kit, c6orf194l ELISA Kit, wu:fj36d09 ELISA Kit, wu:fj49c09 ELISA Kit, 2610020O08Rik ELISA Kit, AA987160 ELISA Kit, AI849147 ELISA Kit, AL024236 ELISA Kit, 2610020o08rik ELISA Kit, nkap ELISA Kit, NKAPL ELISA Kit, NFKB activating protein ELISA Kit, pleckstrin homology and RhoGEF domain containing G5 ELISA Kit, NFKB activating protein S homeolog ELISA Kit, NKAP ELISA Kit, PLEKHG5 ELISA Kit, nkap ELISA Kit, Nkap ELISA Kit, nkap.S ELISA Kit
    背景
    Alternative name: NF Kappa B Activating Protein
    基因ID
    79576
    UniProt
    Q8N5F7
    途径
    Stem Cell Maintenance, The Global Phosphorylation Landscape of SARS-CoV-2 Infection
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