VEGFA CLIA Kit
-
- 抗原 See all VEGFA CLIA Kits
- VEGFA (Vascular Endothelial Growth Factor A (VEGFA))
-
适用
- 人
- 检测方法
- Chemiluminescent
- 实验类型
- Sandwich ELISA
- 检测范围
- 31.25 pg/mL - 2000 pg/mL
- 最低检测浓度
- 31.25 pg/mL
- 应用范围
- ELISA
- 样品类型
- Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Vascular Endothelial Growth Factor A (VEGFA). No significant cross-reactivity or interference between Vascular Endothelial Growth Factor A (VEGFA) and analogues was observed.
- 灵敏度
- 1.17 pg/mL
- Featured
- Discover our best selling VEGFA ELISA Kit
- Top Product
- Discover our top product VEGFA ELISA Kit
-
Vascular Endothelial Growth Factor A (VEGFA) ELISA Kit
VEGFA 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Vascular Endothelial Growth Factor A (VEGFA) ELISA KitVEGFA 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate, Urine
Vascular Endothelial Growth Factor A (VEGFA) ELISA KitVEGFA 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Vascular Endothelial Growth Factor A (VEGFA) ELISA KitVEGFA 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Vascular Endothelial Growth Factor A (VEGFA) ELISA KitVEGFA 适用: 小鸡 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Vascular Endothelial Growth Factor A (VEGFA) ELISA KitVEGFA 适用: Pig Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Vascular Endothelial Growth Factor A (VEGFA) ELISA KitVEGFA 适用: Cow Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Vascular Endothelial Growth Factor A (VEGFA) ELISA KitVEGFA 适用: 犬 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Vascular Endothelial Growth Factor A (VEGFA) ELISA KitVEGFA 适用: 兔 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Vascular Endothelial Growth Factor A (VEGFA) ELISA KitVEGFA 适用: 豚鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
-
- 说明
-
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 2 - 3 h
- 板类型
- Pre-coated
- 实验流程
- The microplate provided in this kit has been pre-coated with an antibody specific to Vascular Endothelial Growth Factor A (VEGFA). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Vascular Endothelial Growth Factor A (VEGFA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Vascular Endothelial Growth Factor A (VEGFA) level in the sample or standard.,
- 实验精密度
-
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vascular Endothelial Growth Factor A (VEGFA) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vascular Endothelial Growth Factor A (VEGFA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
-
- 注意事项
- Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
-
- 抗原 See all VEGFA CLIA Kits
- VEGFA (Vascular Endothelial Growth Factor A (VEGFA))
- 别名
- Vascular Endothelial Growth Factor A (VEGFA 产品)
- 别名
- MVCD1 CLIA Kit, VEGF CLIA Kit, VPF CLIA Kit, Vegf CLIA Kit, Vegf120 CLIA Kit, Vegf164 CLIA Kit, Vegf188 CLIA Kit, Vpf CLIA Kit, vegf CLIA Kit, vegfa CLIA Kit, wu:fj82c06 CLIA Kit, VEGF-A CLIA Kit, VEGF164 CLIA Kit, eVEGF120 CLIA Kit, eVEGF164 CLIA Kit, vegf-a CLIA Kit, vpf CLIA Kit, vefg CLIA Kit, si:dkey-14d8.3 CLIA Kit, wu:fd42e02 CLIA Kit, vascular endothelial growth factor A CLIA Kit, vascular endothelial growth factor Aa CLIA Kit, vascular endothelial growth factor A L homeolog CLIA Kit, vascular endothelial growth factor Ab CLIA Kit, VEGFA CLIA Kit, Vegfa CLIA Kit, vegfaa CLIA Kit, vegfa.L CLIA Kit, vegfa CLIA Kit, vegfab CLIA Kit
- 背景
-
Gene Name: Vascular Endothelial Growth Factor A
Gene Aliases: VEGF, VEGF-A, VPF, Vascular Endothelial Cell Growth Factor A, Vascular Permeability Factor
- 基因ID
- 7422
- UniProt
- P15692
- 途径
- RTK signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
-
