TGFBI CLIA Kit
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北京 101111
Quick Overview for TGFBI CLIA Kit (ABIN5659457)
抗原
See all TGFBI CLIA Kits适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 5.49 pg/mL
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原理
- Mouse Transforming Growth Factor Beta Induced Protein (TGFbI) CLIA Kit for detecting TGFbI in serum, plasma
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Analytical Method
- Quantitative
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特异性
- This assay has high sensitivity and excellent specificity for detection of Transforming Growth Factor Beta Induced Protein (TGFbI). No significant cross-reactivity or interference between Transforming Growth Factor Beta Induced Protein (TGFbI) and analogues was observed.
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灵敏度
- 2.38 pg/mL
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组件
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- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- Substrate A
- Substrate B
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
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试剂未包括
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- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
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Transforming Growth Factor, beta-Induced, 68kDa (TGFBI) ELISA Kit
TGFBI 适用: 人 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Transforming Growth Factor, beta-Induced, 68kDa (TGFBI) ELISA KitTGFBI 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Transforming Growth Factor, beta-Induced, 68kDa (TGFBI) ELISA KitTGFBI 适用: 人 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Transforming Growth Factor, beta-Induced, 68kDa (TGFBI) ELISA KitTGFBI 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum
Transforming Growth Factor, beta-Induced, 68kDa (TGFBI) ELISA KitTGFBI 适用: 大鼠 AA 24-685 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
Transforming Growth Factor, beta-Induced, 68kDa (TGFBI) ELISA KitTGFBI 适用: 人 AA 24-683 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
Transforming Growth Factor, beta-Induced, 68kDa (TGFBI) ELISA KitTGFBI 适用: 小鼠 AA 24-683 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
Transforming Growth Factor, beta-Induced, 68kDa (TGFBI) ELISA KitTGFBI 适用: Pig Colorimetric Sandwich ELISA 0.937 ng/mL - 60 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Transforming Growth Factor, beta-Induced, 68kDa (TGFBI) ELISA KitTGFBI 适用: 人 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Transforming Growth Factor, beta-Induced, 68kDa (TGFBI) ELISA KitTGFBI 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
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样本量
- 100 μL
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实验时间
- 3 h
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板类型
- Pre-coated
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实验流程
- The microplate provided in this kit has been pre-coated with an antibody specific to Transforming Growth Factor Beta Induced Protein (TGFbI). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Transforming Growth Factor Beta Induced Protein (TGFbI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Transforming Growth Factor Beta Induced Protein (TGFbI) level in the sample or standard.,
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实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transforming Growth Factor Beta Induced Protein (TGFbI) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transforming Growth Factor Beta Induced Protein (TGFbI) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% -
限制
- 仅限研究用
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注意事项
- Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
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储存条件
- 4 °C,-20 °C
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储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
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有效期
- 12 months
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- TGFBI (Transforming Growth Factor, beta-Induced, 68kDa (TGFBI))
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别名
- Transforming Growth Factor Beta Induced Protein
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背景
- Gene Aliases: TGF-BI, CSD3, LCD1, CSD1, CSD2, BIGH3, CDB1, CDGG1, RGD-CAP, Kerato-Epithelin, RGD-Containing Collagen-Associated Protein
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基因ID
- 21810
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UniProt
- P82198
抗原 See all TGFBI CLIA Kits
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