TGFBI ELISA 试剂盒
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Quick Overview for TGFBI ELISA 试剂盒 (ABIN1889371)
抗原
See all TGFBI ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 62.5 pg/mL
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原理
- Sandwich Human beta IG-H3/TGFBI ELISA Kit to quantitate Human TGFBI in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: G24-H683Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Transforming Growth Factor, beta-Induced, 68kDa (TGFBI) ELISA Kit
TGFBI 适用: 人 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Transforming Growth Factor, beta-Induced, 68kDa (TGFBI) ELISA KitTGFBI 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Transforming Growth Factor, beta-Induced, 68kDa (TGFBI) ELISA KitTGFBI 适用: 人 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Transforming Growth Factor, beta-Induced, 68kDa (TGFBI) ELISA KitTGFBI 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum
Transforming Growth Factor, beta-Induced, 68kDa (TGFBI) ELISA KitTGFBI 适用: 小鼠 AA 24-683 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
Transforming Growth Factor, beta-Induced, 68kDa (TGFBI) ELISA KitTGFBI 适用: 大鼠 AA 24-685 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
Transforming Growth Factor, beta-Induced, 68kDa (TGFBI) ELISA KitTGFBI 适用: Pig Colorimetric Sandwich ELISA 0.937 ng/mL - 60 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Transforming Growth Factor, beta-Induced, 68kDa (TGFBI) ELISA KitTGFBI 适用: 人 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Transforming Growth Factor, beta-Induced, 68kDa (TGFBI) ELISA KitTGFBI 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum
Transforming Growth Factor, beta-Induced, 68kDa (TGFBI) ELISA KitTGFBI 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 4.4%, 7.3%, 5.8%
Inter-Assay Precision (CV%): 4.6%, 9.1%, 5.8% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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: "Treg/IL-17 ratio and Treg differentiation in patients with COPD." in: PLoS ONE, Vol. 9, Issue 10, pp. e111044, (2014) (PubMed).
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- TGFBI (Transforming Growth Factor, beta-Induced, 68kDa (TGFBI))
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别名
- TGFBI
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背景
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Background: Transforming growth factor, beta-induced, 68 kDa, also known as TGFBI (initially called BIGH3, BIG-H3), is a protein which in humans is encoded by the TGFBI gene. It is mapped to 5q31.1. This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion.
Gene Full Name: transforming growth factor beta induced
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基因ID
- 7045
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UniProt
- Q15582
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