TMSB4X ELISA 试剂盒
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Quick Overview for TMSB4X ELISA 试剂盒 (ABIN5659216)
抗原
See all TMSB4X ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 2.47 ng/mL
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原理
- Human Thymosin Beta 4 (TMSB4X) ELISA Kit for detecting TMSB4X in serum, plasma, tissue homogenates, cell lysates, cell culture supernates
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Analytical Method
- Quantitative
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特异性
- This assay has high sensitivity and excellent specificity for detection of Thymosin Beta 4 (Tb4). No significant cross-reactivity or interference between Thymosin Beta 4 (Tb4) and analogues was observed.
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灵敏度
- 0.92 ng/mL
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组件
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- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- TMB Substrate
- Stop Solution
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
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试剂未包括
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- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
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Thymosin beta-4 (TMSB4X) ELISA Kit
TMSB4X 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Thymosin beta-4 (TMSB4X) ELISA KitTMSB4X 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Thymosin beta-4 (TMSB4X) ELISA KitTMSB4X 适用: 人 Colorimetric Competition ELISA 2.47 ng/mL - 200 ng/mL Plasma, Serum
Thymosin beta-4 (TMSB4X) ELISA KitTMSB4X 适用: 小鼠 Colorimetric Competition ELISA 0.5-10 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Thymosin beta-4 (TMSB4X) ELISA KitTMSB4X 适用: 人, 大鼠, 小鼠 Colorimetric Competition ELISA 0.1 ng/mL - 1000 ng/mL Plasma, Serum
Thymosin beta-4 (TMSB4X) ELISA KitTMSB4X 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Samples, Tissue Lysate
Thymosin beta-4 (TMSB4X) ELISA KitTMSB4X 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
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样本量
- 50 μL
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实验时间
- 2 h
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板类型
- Pre-coated
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实验流程
- This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Thymosin Beta 4 (Tb4) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Thymosin Beta 4 (Tb4) and unlabeled Thymosin Beta 4 (Tb4) (Standards or samples) with the pre-coated antibody specific to Thymosin Beta 4 (Tb4). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Thymosin Beta 4 (Tb4) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Thymosin Beta 4 (Tb4) in the sample.
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实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thymosin Beta 4 (Tb4) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thymosin Beta 4 (Tb4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% -
限制
- 仅限研究用
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注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
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储存条件
- 4 °C,-20 °C
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储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
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有效期
- 12 months
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- TMSB4X (Thymosin beta-4 (TMSB4X))
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别名
- Thymosin Beta 4
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背景
- Gene Aliases: Tb4, FX, PTMB4, TB4X, TMSB4, Thymosin,Beta 4,X-Linked, TB-500, Seraspenide, Hematopoietic system regulatory peptide
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基因ID
- 7114
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UniProt
- P62328
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途径
- Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Maintenance of Protein Location, Smooth Muscle Cell Migration
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