TMSB4X ELISA 试剂盒
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Quick Overview for TMSB4X ELISA 试剂盒 (ABIN5526754)
抗原
See all TMSB4X ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.1 ng/mL
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原理
- Human/Mouse/Rat Thymosin beta-4 EIA Kit optimized for serum, plasma and cell culture media. Competition-based ELISA
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Analytical Method
- Quantitative
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特异性
- This EIA kit is designed to detect human, mouse, and rat Thymosin-b4
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灵敏度
- 1.1 ng/mL
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产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Standard Peptide
- Assay Diluent(s)
- Biotinylated Peptide
- HRP-Streptavidin
- TMB One-Step Substrate
- Stop Solution
- Assay Diagram
- Positive Control Sample
- Capture Antibody
- User Manual
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Standard Peptide
- Assay Diluent(s)
- Biotinylated Peptide
- HRP-Streptavidin
- TMB One-Step Substrate
- Stop Solution
- Assay Diagram
- Positive Control Sample
- Capture Antibody
- User Manual
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试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Orbital shaker
- Aluminum foil
- Saran Wrap
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- SigmaPlot software (or other software that can perform four-parameter logistic regression models)
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Thymosin beta-4 (TMSB4X) ELISA Kit
TMSB4X 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Thymosin beta-4 (TMSB4X) ELISA KitTMSB4X 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Thymosin beta-4 (TMSB4X) ELISA KitTMSB4X 适用: 人 Colorimetric Competition ELISA 2.47 ng/mL - 200 ng/mL Plasma, Serum
Thymosin beta-4 (TMSB4X) ELISA KitTMSB4X 适用: 小鼠 Colorimetric Competition ELISA 0.5-10 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Thymosin beta-4 (TMSB4X) ELISA KitTMSB4X 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Samples, Tissue Lysate
Thymosin beta-4 (TMSB4X) ELISA KitTMSB4X 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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应用备注
- Suggested Plasma Dilution: Human: 2x / Mouse: 2x / Rat: 2x
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed.
- Add 100 μL detection antibody to each well.
- Incubate 1.5 h at RT or O/N at 4°C.
- Add 100 μL standard or sample to each well.
- Incubate 2.5 h at RT.
- Add 100 μL prepared streptavidin solution.
- Incubate 45 min at RT.
- Add 100 μL TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL Stop Solution to each well.
- Read plate at 450 nm immediately.
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试剂准备
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Recommended Dilution for serum and plasma samplesHuman: 2x / Mouse: 2x / Rat: 2x
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened microplate wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.
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有效期
- 6 months
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- TMSB4X (Thymosin beta-4 (TMSB4X))
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别名
- Thymosin beta-4
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背景
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Gene Symbols: TMSB4X, TB4X, THYB4, TMSB4
Background: Thymosin beta-4 is a 43 amino acids protein that is encoded by the TMSB4X gene. It plays a role in regulation of actin polymerization. Thymosin beta-4 binds to and sequesters actin monomers (G actin) and therefore inhibits actin polymerization. It is also involved in cell proliferation, migration, and differentiation. It has been studied in a number of clinical trials.
Protein Name (Synonyms): Thymosin beta-4 (T beta-4) (Fx) [Cleaved into: Hematopoietic system regulatory peptide (Seraspenide)]
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基因ID
- 7114
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UniProt
- P62328
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途径
- Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Maintenance of Protein Location, Smooth Muscle Cell Migration
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