TEK ELISA 试剂盒
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- 抗原 See all TEK ELISA试剂盒
- TEK (TEK Tyrosine Kinase, Endothelial (TEK))
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适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.312 ng/mL - 20 ng/mL
- 最低检测浓度
- 0.312 ng/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of TEK Tyrosine Kinase, Endothelial (Tie2). No significant cross-reactivity or interference between TEK Tyrosine Kinase, Endothelial (Tie2) and analogues was observed.
- 灵敏度
- 0.135 ng/mL
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TEK Tyrosine Kinase, Endothelial (TEK) ELISA Kit
TEK 适用: 人 Colorimetric Sandwich ELISA 0.94 ng/mL - 60 ng/mL Plasma, Serum, Tissue Homogenate
TEK Tyrosine Kinase, Endothelial (TEK) ELISA KitTEK 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
TEK Tyrosine Kinase, Endothelial (TEK) ELISA KitTEK 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
TEK Tyrosine Kinase, Endothelial (TEK) ELISA KitTEK 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
TEK Tyrosine Kinase, Endothelial (TEK) ELISA KitTEK 适用: 人 AA 23-745 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
TEK Tyrosine Kinase, Endothelial (TEK) ELISA KitTEK 适用: 小鼠 AA 23-744 Colorimetric Sandwich ELISA 125-8000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum, Tissue Homogenate
TEK Tyrosine Kinase, Endothelial (TEK) ELISA KitTEK 适用: 小鼠 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Tissue Homogenate
TEK Tyrosine Kinase, Endothelial (TEK) ELISA KitTEK 适用: 人 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TEK Tyrosine Kinase, Endothelial (Tie2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to TEK Tyrosine Kinase, Endothelial (Tie2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TEK Tyrosine Kinase, Endothelial (Tie2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TEK Tyrosine Kinase, Endothelial (Tie2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TEK Tyrosine Kinase, Endothelial (Tie2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TEK Tyrosine Kinase, Endothelial (Tie2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all TEK ELISA试剂盒
- TEK (TEK Tyrosine Kinase, Endothelial (TEK))
- 别名
- TEK Tyrosine Kinase, Endothelial (TEK 产品)
- 别名
- TEK ELISA Kit, DKFZp469E064 ELISA Kit, AA517024 ELISA Kit, Cd202b ELISA Kit, Hyk ELISA Kit, Tie2 ELISA Kit, tie-2 ELISA Kit, CD202B ELISA Kit, TIE-2 ELISA Kit, TIE2 ELISA Kit, VMCM ELISA Kit, VMCM1 ELISA Kit, Tie-2 ELISA Kit, tie2 ELISA Kit, TEK receptor tyrosine kinase ELISA Kit, angiopoietin-1 receptor ELISA Kit, TEK tyrosine kinase, endothelial ELISA Kit, TEK ELISA Kit, LOC100408334 ELISA Kit, tek ELISA Kit, Tek ELISA Kit
- 背景
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Gene Name: TEK Tyrosine Kinase, Endothelial
Gene Aliases: CD202b, CD202-B, TEK, VMCM1, Tyrosine Kinase With Immunoglobulin Like And EGF Like Domains 2, Venous Malformations Multiple Cutaneous And Mucosal
- 基因ID
- 89804
- UniProt
- D3ZCD0
- 途径
- RTK signaling, Growth Factor Binding
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