KIT CLIA Kit
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- 抗原 See all KIT CLIA Kits
- KIT (Mast/stem Cell Growth Factor Receptor (KIT))
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适用
- 人
- 检测方法
- Chemiluminescent
- 实验类型
- Sandwich ELISA
- 检测范围
- 468.75 pg/mL - 30000 pg/mL
- 最低检测浓度
- 468.75 pg/mL
- 应用范围
- ELISA
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Stem Cell Factor Receptor (SCFR). No significant cross-reactivity or interference between Stem Cell Factor Receptor (SCFR) and analogues was observed.
- 灵敏度
- 15.3 pg/mL
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Mast/stem Cell Growth Factor Receptor (KIT) ELISA Kit
KIT 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Mast/stem Cell Growth Factor Receptor (KIT) ELISA KitKIT 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Mast/stem Cell Growth Factor Receptor (KIT) ELISA KitKIT 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum
Mast/stem Cell Growth Factor Receptor (KIT) ELISA KitKIT 适用: 人 AA 26-520 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Mast/stem Cell Growth Factor Receptor (KIT) ELISA KitKIT 适用: 小鼠 AA 25-527 Colorimetric Sandwich ELISA
Mast/stem Cell Growth Factor Receptor (KIT) ELISA KitKIT 适用: 小鼠 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 2 - 3 h
- 板类型
- Pre-coated
- 实验流程
- The microplate provided in this kit has been pre-coated with an antibody specific to Stem Cell Factor Receptor (SCFR). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Stem Cell Factor Receptor (SCFR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Stem Cell Factor Receptor (SCFR) level in the sample or standard.,
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Stem Cell Factor Receptor (SCFR) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Stem Cell Factor Receptor (SCFR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all KIT CLIA Kits
- KIT (Mast/stem Cell Growth Factor Receptor (KIT))
- 别名
- Stem Cell Factor Receptor (KIT 产品)
- 别名
- C-Kit CLIA Kit, CD117 CLIA Kit, PBT CLIA Kit, SCFR CLIA Kit, Bs CLIA Kit, Fdc CLIA Kit, Gsfsco1 CLIA Kit, Gsfsco5 CLIA Kit, Gsfsow3 CLIA Kit, SCO1 CLIA Kit, SCO5 CLIA Kit, SOW3 CLIA Kit, Ssm CLIA Kit, Tr-kit CLIA Kit, W CLIA Kit, c-KIT CLIA Kit, c-kit CLIA Kit, MGF CLIA Kit, KIT CLIA Kit, C-KIT CLIA Kit, kit CLIA Kit, receptor CLIA Kit, sparse CLIA Kit, Xkl-1 CLIA Kit, kit-a CLIA Kit, kit-b CLIA Kit, kl1-A CLIA Kit, krk1 CLIA Kit, pbt CLIA Kit, scfr CLIA Kit, KIT proto-oncogene receptor tyrosine kinase CLIA Kit, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog a CLIA Kit, KIT proto-oncogene receptor tyrosine kinase S homeolog CLIA Kit, KIT CLIA Kit, Kit CLIA Kit, kita CLIA Kit, kit CLIA Kit, kit.S CLIA Kit
- 背景
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Gene Name: Stem Cell Factor Receptor
Gene Aliases: CD117, KIT, C-Kit, PBT, p145 c-Kit, C-Kit Receptor, Tyrosine-Protein Kinase, V-Kit Hardy-Zuckerman 4 Feline Sarcoma Viral Oncogene Homolog, Piebald Trait
- 基因ID
- 3815
- UniProt
- P10721
- 途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Sensory Perception of Sound, Stem Cell Maintenance, Production of Molecular Mediator of Immune Response, Regulation of long-term Neuronal Synaptic Plasticity
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