PDGFB CLIA Kit
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- 抗原 See all PDGFB CLIA Kits
- PDGFB (Platelet Derived Growth Factor Subunit B (PDGFB))
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适用
- 人
- 检测方法
- Chemiluminescent
- 实验类型
- Sandwich ELISA
- 检测范围
- 31.25 pg/mL - 2000 pg/mL
- 最低检测浓度
- 31.25 pg/mL
- 应用范围
- ELISA
- 样品类型
- Plasma
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Platelet Derived Growth Factor Subunit B (PDGFB). No significant cross-reactivity or interference between Platelet Derived Growth Factor Subunit B (PDGFB) and analogues was observed.
- 灵敏度
- 1.26 pg/mL
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Platelet Derived Growth Factor Subunit B (PDGFB) ELISA Kit
PDGFB 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Platelet Derived Growth Factor Subunit B (PDGFB) ELISA KitPDGFB 适用: 大鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Platelet Derived Growth Factor Subunit B (PDGFB) ELISA KitPDGFB 适用: 小鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Platelet Derived Growth Factor Subunit B (PDGFB) ELISA KitPDGFB 适用: Pig Colorimetric Sandwich ELISA 46.88 pg/mL - 3000 pg/mL Plasma, Serum, Tissue Homogenate
Platelet Derived Growth Factor Subunit B (PDGFB) ELISA KitPDGFB 适用: Cow Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 2 - 3 h
- 板类型
- Pre-coated
- 实验流程
- The microplate provided in this kit has been pre-coated with an antibody specific to Platelet Derived Growth Factor Subunit B (PDGFB). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Platelet Derived Growth Factor Subunit B (PDGFB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Platelet Derived Growth Factor Subunit B (PDGFB) level in the sample or standard.,
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Platelet Derived Growth Factor Subunit B (PDGFB) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Platelet Derived Growth Factor Subunit B (PDGFB) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all PDGFB CLIA Kits
- PDGFB (Platelet Derived Growth Factor Subunit B (PDGFB))
- 别名
- Platelet Derived Growth Factor Subunit B (PDGFB 产品)
- 别名
- PDGF-2 CLIA Kit, PDGF2 CLIA Kit, SIS CLIA Kit, SSV CLIA Kit, c-sis CLIA Kit, pdgf2 CLIA Kit, sis CLIA Kit, si:ch211-79m20.1 CLIA Kit, si:dkey-261d11.1 CLIA Kit, PDGF-B CLIA Kit, Sis CLIA Kit, platelet derived growth factor subunit B CLIA Kit, platelet-derived growth factor beta polypeptide CLIA Kit, platelet derived growth factor subunit B S homeolog CLIA Kit, platelet-derived growth factor beta polypeptide a CLIA Kit, platelet derived growth factor, B polypeptide CLIA Kit, PDGFB CLIA Kit, pdgfb.S CLIA Kit, pdgfba CLIA Kit, Pdgfb CLIA Kit
- 背景
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Gene Name: Platelet Derived Growth Factor Subunit B
Gene Aliases: PDGF-B, PDGF2, SIS, SSV, C-sis, Simian Sarcoma Viral(v-sis)Oncogene Homolog, Platelet Derived Growth Factor Beta Polypeptide, Proto-oncogene c-Sis, Becaplermin
- 基因ID
- 5155
- UniProt
- P01127
- 途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling
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