Neuregulin 1 ELISA 试剂盒
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- 抗原 See all Neuregulin 1 (NRG1) ELISA试剂盒
- Neuregulin 1 (NRG1)
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 15.62 pg/mL - 1000 pg/mL
- 最低检测浓度
- 15.62 pg/mL
- 应用范围
- ELISA
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Neuregulin 1 (NRG1). No significant cross-reactivity or interference between Neuregulin 1 (NRG1) and analogues was observed.
- 灵敏度
- 6.2 pg/mL
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Neuregulin 1 (NRG1) ELISA Kit
NRG1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Neuregulin 1 (NRG1) ELISA KitNRG1 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Neuregulin 1 (NRG1) ELISA KitNRG1 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Neuregulin 1 (NRG1) ELISA KitNRG1 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Neuregulin 1 (NRG1) ELISA KitWide Range NRG1 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Neuregulin 1 (NRG1) ELISA KitNRG1 适用: 人 AA 2-246 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neuregulin 1 (NRG1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neuregulin 1 (NRG1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neuregulin 1 (NRG1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neuregulin 1 (NRG1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neuregulin 1 (NRG1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neuregulin 1 (NRG1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all Neuregulin 1 (NRG1) ELISA试剂盒
- Neuregulin 1 (NRG1)
- 别名
- Neuregulin 1 (NRG1 产品)
- 别名
- 6030402G23Rik ELISA Kit, ARIA ELISA Kit, D230005F13Rik ELISA Kit, GGF ELISA Kit, GGFII ELISA Kit, HRG ELISA Kit, HRGalpha ELISA Kit, Hgl ELISA Kit, NDF ELISA Kit, SMDF ELISA Kit, GGF2 ELISA Kit, HGL ELISA Kit, HRG1 ELISA Kit, HRGA ELISA Kit, MST131 ELISA Kit, NRG-1 ELISA Kit, heregulin ELISA Kit, nrg1-A ELISA Kit, pro-neuregulin-1 ELISA Kit, NRG1 ELISA Kit, wu:fm70e01 ELISA Kit, DKFZp459A1838 ELISA Kit, neuregulin 1 ELISA Kit, histidine rich glycoprotein ELISA Kit, neuregulin 1 L homeolog ELISA Kit, Nrg1 ELISA Kit, NRG1 ELISA Kit, HRG ELISA Kit, nrg1.L ELISA Kit, nrg1 ELISA Kit
- 背景
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Gene Name: Neuregulin 1
Gene Aliases: ARIA, GGF, HGL, HRG, HRGA, NDF, SMDF, Acetylcholine receptor-inducing activity, Glial growth factor, Heregulin, Neu differentiation factor, Sensory and motor neuron-derived factor
- 基因ID
- 211323
- UniProt
- Q6DR98
- 途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation
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