MIF CLIA Kit
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北京 101111
Quick Overview for MIF CLIA Kit (ABIN5656602)
抗原
See all MIF CLIA Kits适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156.25 pg/mL
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Analytical Method
- Quantitative
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特异性
- This assay has high sensitivity and excellent specificity for detection of Macrophage Migration Inhibitory Factor (MIF). No significant cross-reactivity or interference between Macrophage Migration Inhibitory Factor (MIF) and analogues was observed.
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灵敏度
- 6.4 pg/mL
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Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) ELISA Kit
MIF 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) ELISA KitMIF 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) ELISA KitMIF 适用: 大鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) ELISA KitWide Range MIF 适用: 大鼠 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) ELISA KitMIF 适用: 人 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) ELISA KitMIF 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) ELISA KitMIF 适用: 兔 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) ELISA KitMIF 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) ELISA KitMIF 适用: 人 AA 2-115 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Milk, Plasma (EDTA), Plasma (heparin), Serum
Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) ELISA KitMIF 适用: 小鼠 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Plasma, Serum
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说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
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实验时间
- 2 - 3 h
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板类型
- Pre-coated
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实验流程
- The microplate provided in this kit has been pre-coated with an antibody specific to Macrophage Migration Inhibitory Factor (MIF). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Macrophage Migration Inhibitory Factor (MIF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Macrophage Migration Inhibitory Factor (MIF) level in the sample or standard.,
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实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Macrophage Migration Inhibitory Factor (MIF) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Macrophage Migration Inhibitory Factor (MIF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% -
限制
- 仅限研究用
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注意事项
- Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
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储存条件
- 4 °C,-20 °C
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储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
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有效期
- 4-8 months
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- MIF (Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF))
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别名
- Macrophage Migration Inhibitory Factor
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背景
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Gene Name: Macrophage Migration Inhibitory Factor
Gene Aliases: GIF, GLIF, MMIF, Glycosylation-Inhibiting Factor, L-dopachrome isomerase, L-dopachrome tautomerase, Phenylpyruvate tautomerase
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基因ID
- 17319
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UniProt
- P34884
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途径
- Regulation of Systemic Arterial Blood Pressure by Hormones, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling
抗原 See all MIF CLIA Kits
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