MIF ELISA 试剂盒
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Quick Overview for MIF ELISA 试剂盒 (ABIN411399)
抗原
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适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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原理
- Sandwich Human MIF ELISA Kit to quantitate Human MIF in cell culture supernatants, serum, plasma (heparin, EDTA) and human milk.
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: E.coli
Immunogen sequence: P2-A115Capture antibody and Detection antibody: polyclonal antibody from goat, polyclonal antibody from goat
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 20 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) ELISA Kit
MIF 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) ELISA KitMIF 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) ELISA KitMIF 适用: 大鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) ELISA KitMIF 适用: 人 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) ELISA KitMIF 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) ELISA KitMIF 适用: 兔 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) ELISA KitMIF 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) ELISA KitWide Range MIF 适用: 大鼠 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) ELISA KitMIF 适用: 小鼠 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Plasma, Serum
Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) ELISA KitMIF 适用: 猴 AA 2-115 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Milk, Plasma (EDTA), Plasma (heparin), Serum
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 4.3%, 4%, 4.5%
Inter-Assay Precision (CV%): 5.4%, 4.6%, 6.3% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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: "Macrophage migration inhibitory factor as an incriminating agent in vitiligo." in: Anais brasileiros de dermatologia, Vol. 93, Issue 2, pp. 191-196, (2018) (PubMed).
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- MIF (Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF))
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别名
- MIF
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背景
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Background: Macrophage migration inhibitory factor (MIF) is a protein which in humans is encoded by the MIF gene.1, 2 This gene is located to human chromosome 22q11.2.3 It is remarkably small, it has 3 exons separated by introns of only 189 and 95 bp, and covers less than 1 kb.4 This gene encodes a lymphokine that may be involved in cell-mediated immunity, immunoregulation, and inflammation.5 MIF plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein might form a complex in the cytosol near the peripheral plasma membrane, which may possibly indicate a role in integrin signaling pathways. MIF also plays a central role in the toxic response to endotoxemia and possibly septic shock.Macrophage migration inhibitory factor has been reported to interact with COP9 constitutive photomorphogenic homolog subunit 5, CD74, BNIPL, and CXCR4.
Gene Full Name: macrophage migration inhibitory factor
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基因ID
- 4282
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UniProt
- P14174
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途径
- Regulation of Systemic Arterial Blood Pressure by Hormones, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling
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