IFNA2 CLIA Kit
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北京 101111
Quick Overview for IFNA2 CLIA Kit (ABIN5655566)
抗原
See all IFNA2 CLIA Kits适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 1.37 pg/mL
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原理
- Human Interferon Alpha 2 (IFNa2) CLIA Kit for detecting IFNa2 in serum, plasma, tissue homogenates, cell lysates, cell culture supernates
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Analytical Method
- Quantitative
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特异性
- This assay has high sensitivity and excellent specificity for detection of Interferon Alpha 2 (IFNa2). No significant cross-reactivity or interference between Interferon Alpha 2 (IFNa2) and analogues was observed.
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灵敏度
- 0.49 pg/mL
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组件
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- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- Substrate A
- Substrate B
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
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试剂未包括
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- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
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Interferon, alpha 2 (IFNA2) ELISA Kit
IFNA2 适用: 小鼠 Colorimetric Sandwich ELISA 9.38 pg/mL - 600 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Interferon, alpha 2 (IFNA2) ELISA KitIFNA2 适用: 小鼠 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Interferon, alpha 2 (IFNA2) ELISA KitIFNA2 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interferon, alpha 2 (IFNA2) ELISA KitIFNA2 适用: 人 Colorimetric 31.25-2000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Interferon, alpha 2 (IFNA2) ELISA KitIFNA2 适用: 人 AA 24-189 Colorimetric Sandwich ELISA 3.12 pg/mL - 200 pg/mL Cell Culture Supernatant, Serum
Interferon, alpha 2 (IFNA2) ELISA KitIFNA2 适用: 小鼠 Colorimetric Sandwich ELISA 9.38-600 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Interferon, alpha 2 (IFNA2) ELISA KitIFNA2 适用: 人 Colorimetric Sandwich ELISA 31.25-2000 pg/mL Cell Culture Cells, Plasma, Serum
Interferon, alpha 2 (IFNA2) ELISA KitIFNA2 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interferon, alpha 2 (IFNA2) ELISA KitIFNA2 适用: 小鼠 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
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样本量
- 100 μL
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实验时间
- 3 h
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板类型
- Pre-coated
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实验流程
- The microplate provided in this kit has been pre-coated with an antibody specific to Interferon Alpha 2 (IFNa2). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Interferon Alpha 2 (IFNa2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Interferon Alpha 2 (IFNa2) level in the sample or standard.,
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实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interferon Alpha 2 (IFNa2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interferon Alpha 2 (IFNa2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% -
限制
- 仅限研究用
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注意事项
- Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
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储存条件
- 4 °C,-20 °C
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储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
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有效期
- 12 months
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- IFNA2 (Interferon, alpha 2 (IFNA2))
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别名
- Interferon Alpha 2
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背景
- Gene Aliases: IFNA2A, IFNA2B, IFNA2C, LeIF A, Alpha-2a Interferon, Interferon Alpha 2b, Interferon Alpha A
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基因ID
- 3440
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UniProt
- P01563
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途径
- JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Hepatitis C
抗原 See all IFNA2 CLIA Kits
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