IFNA2 ELISA 试剂盒
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北京 101111
Quick Overview for IFNA2 ELISA 试剂盒 (ABIN8055732)
抗原
See all IFNA2 ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 3.12 pg/mL
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原理
- Sandwich Human IFNA2 ELISA Kit to quantitate Human IFNA2 in cell culture supernatants and serum.
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: E.coli
Immunogen sequence: C24-E189Capture antibody and Detection antibody: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 1 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Interferon, alpha 2 (IFNA2) ELISA Kit
IFNA2 适用: 小鼠 Colorimetric Sandwich ELISA 9.38 pg/mL - 600 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Interferon, alpha 2 (IFNA2) ELISA KitIFNA2 适用: 小鼠 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Interferon, alpha 2 (IFNA2) ELISA KitIFNA2 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interferon, alpha 2 (IFNA2) ELISA KitIFNA2 适用: 人 Colorimetric 31.25-2000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Interferon, alpha 2 (IFNA2) ELISA KitIFNA2 适用: 小鼠 Colorimetric Sandwich ELISA 9.38-600 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Interferon, alpha 2 (IFNA2) ELISA KitIFNA2 适用: 人 Colorimetric Sandwich ELISA 31.25-2000 pg/mL Cell Culture Cells, Plasma, Serum
Interferon, alpha 2 (IFNA2) CLIA KitIFNA2 适用: 人 Chemiluminescent Sandwich ELISA 1.37 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interferon, alpha 2 (IFNA2) ELISA KitIFNA2 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 4.2%, 4.3%, 3.9%
Inter-Assay Precision (CV%): 5.1%, 5.9%, 5.7% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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- IFNA2 (Interferon, alpha 2 (IFNA2))
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别名
- IFNA2
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背景
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Background: Interferon alpha-2 is a protein that in humans is encoded by the IFNA2 gene. This gene is a member of the alpha interferon gene cluster on chromosome 9. The encoded cytokine is a member of the type I interferon family that is produced in response to viral infection as a key part of the innate immune response with potent antiviral, antiproliferative and immunomodulatory properties. This cytokine, like other type I interferons, binds a plasma membrane receptor made of IFNAR1 and IFNAR2 that is ubiquitously expressed, and thus is able to act on virtually all body cells. The encoded protein is effective in reducing the symptoms and duration of the common cold and in treating many types of cancer, including some hematological malignancies and solid tumors. A deficiency of type I interferon in the blood is thought to be a hallmark of severe COVID-19 and may provide a rationale for a combined therapeutic approach.
Gene Full Name: interferon alpha 2
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基因ID
- 3440
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UniProt
- P01563
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途径
- JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Hepatitis C
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