HMGB1 CLIA Kit
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- 抗原 See all HMGB1 CLIA Kits
- HMGB1 (High Mobility Group Box 1 (HMGB1))
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适用
- 人
- 检测方法
- Chemiluminescent
- 实验类型
- Sandwich ELISA
- 检测范围
- 156.25 pg/mL - 10000 pg/mL
- 最低检测浓度
- 156.25 pg/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of High Mobility Group Protein 1 (HMG1). No significant cross-reactivity or interference between High Mobility Group Protein 1 (HMG1) and analogues was observed.
- 灵敏度
- 5.2 pg/mL
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 2 - 3 h
- 板类型
- Pre-coated
- 实验流程
- The microplate provided in this kit has been pre-coated with an antibody specific to High Mobility Group Protein 1 (HMG1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to High Mobility Group Protein 1 (HMG1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the High Mobility Group Protein 1 (HMG1) level in the sample or standard.,
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Mobility Group Protein 1 (HMG1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Mobility Group Protein 1 (HMG1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all HMGB1 CLIA Kits
- HMGB1 (High Mobility Group Box 1 (HMGB1))
- 别名
- High Mobility Group Protein 1 (HMGB1 产品)
- 别名
- HMG1 CLIA Kit, HMG3 CLIA Kit, SBP-1 CLIA Kit, DEF CLIA Kit, HMG-1 CLIA Kit, Hmg1 CLIA Kit, amphoterin CLIA Kit, p30 CLIA Kit, hmgb1 CLIA Kit, ik:tdsubc_1a5 CLIA Kit, wu:fb23c02 CLIA Kit, xx:tdsubc_1a5 CLIA Kit, zgc:56110 CLIA Kit, zgc:77104 CLIA Kit, hmg-1 CLIA Kit, hmg3 CLIA Kit, sbp-1 CLIA Kit, hmg1 CLIA Kit, HMGB1 CLIA Kit, Ac2-008 CLIA Kit, high mobility group box 1 CLIA Kit, high-mobility group box 1 CLIA Kit, high mobility group box 1a CLIA Kit, high mobility group box 1 L homeolog CLIA Kit, high mobility group protein B1 CLIA Kit, HMGB1 CLIA Kit, Hmgb1 CLIA Kit, hmgb1 CLIA Kit, hmgb1a CLIA Kit, hmgb1.L CLIA Kit, LOC100359149 CLIA Kit
- 背景
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Gene Name: High Mobility Group Protein 1
Gene Aliases: HMGB1, HMG3, SBP1, Sulfoglucuronyl Carbohydrate Binding Protein, Amphoterin, High Mobility Group Box 1 Protein
- 基因ID
- 3146
- UniProt
- P09429
- 途径
- p53 Pathway, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome
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