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Hexokinase 1 ELISA 试剂盒

HK1 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
产品编号 ABIN5655112
发货至: 中国
  • 抗原 See all Hexokinase 1 (HK1) ELISA试剂盒
    Hexokinase 1 (HK1)
    适用
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    62.5 pg/mL - 4000 pg/mL
    最低检测浓度
    62.5 pg/mL
    应用范围
    ELISA
    样品类型
    Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    特异性
    This assay has high sensitivity and excellent specificity for detection of Hexokinase 1 (HK1). No significant cross-reactivity or interference between Hexokinase 1 (HK1) and analogues was observed.
    灵敏度
    27.5 pg/mL
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  • 说明

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    实验时间
    3 h
    板类型
    Pre-coated
    实验流程
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hexokinase 1 (HK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hexokinase 1 (HK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hexokinase 1 (HK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hexokinase 1 (HK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    实验精密度
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hexokinase 1 (HK1) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hexokinase 1 (HK1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    限制
    仅限研究用
  • 注意事项
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    储存条件
    4 °C,-20 °C
    储存方法
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    有效期
    4-8 months
  • 抗原 See all Hexokinase 1 (HK1) ELISA试剂盒
    Hexokinase 1 (HK1)
    别名
    Hexokinase 1 (HK1 产品)
    别名
    HK1-ta ELISA Kit, HK1-tb ELISA Kit, HK1-tc ELISA Kit, HKD ELISA Kit, HKI ELISA Kit, HMSNR ELISA Kit, HXK1 ELISA Kit, Hexokinase ELISA Kit, hk1 ELISA Kit, HEX ELISA Kit, HEX-1 ELISA Kit, HK ELISA Kit, ARABIDOPSIS THALIANA HEXOKINASE 1 ELISA Kit, ATHXK1 ELISA Kit, F19B15.160 ELISA Kit, F19B15_160 ELISA Kit, GIN2 ELISA Kit, GLUCOSE INSENSITIVE 2 ELISA Kit, HEXOKINASE ELISA Kit, hexokinase 1 ELISA Kit, im:7148527 ELISA Kit, wu:fc09d08 ELISA Kit, wu:fc16e02 ELISA Kit, wu:fc21e02 ELISA Kit, wu:fq14b11 ELISA Kit, zgc:55790 ELISA Kit, zgc:77618 ELISA Kit, hexokinase-1 ELISA Kit, HK1 ELISA Kit, BB404130 ELISA Kit, Hk-1 ELISA Kit, Hk1-s ELISA Kit, dea ELISA Kit, mHk1-s ELISA Kit, HEXOKIN ELISA Kit, Hxk1 ELISA Kit, NtHxK1 ELISA Kit, NtHxk ELISA Kit, hexokinase ELISA Kit, StHK1 ELISA Kit, hxk ELISA Kit, hk1-A ELISA Kit, hexokinase 1 ELISA Kit, hexokinase-1 ELISA Kit, Hexokinase 1 ELISA Kit, hexokinase 1, putative ELISA Kit, hexokinase 1 L homeolog ELISA Kit, HK1 ELISA Kit, hk1 ELISA Kit, LOC100136279 ELISA Kit, Hex1 ELISA Kit, HXK1 ELISA Kit, Hk1 ELISA Kit, TA19800 ELISA Kit, hxk1 ELISA Kit, LOC107797523 ELISA Kit, LOC102604144 ELISA Kit, hk1.L ELISA Kit
    背景

    Gene Name: Hexokinase 1

    Gene Aliases: HK1-Ta, HK1-Tb, HK1-Tc, HKI, HXK1, Brain form hexokinase

    基因ID
    3098
    UniProt
    P19367
    途径
    Carbohydrate Homeostasis, Warburg Effect
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