HBEGF CLIA Kit
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- 抗原 See all HBEGF CLIA Kits
- HBEGF (Heparin-Binding EGF-Like Growth Factor (HBEGF))
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适用
- 大鼠
- 检测方法
- Chemiluminescent
- 实验类型
- Sandwich ELISA
- 检测范围
- 15.62 pg/mL - 1000 pg/mL
- 最低检测浓度
- 15.62 pg/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF). No significant cross-reactivity or interference between Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) and analogues was observed.
- 灵敏度
- 0.55 pg/mL
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 2 - 3 h
- 板类型
- Pre-coated
- 实验流程
- The microplate provided in this kit has been pre-coated with an antibody specific to Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) level in the sample or standard.,
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all HBEGF CLIA Kits
- HBEGF (Heparin-Binding EGF-Like Growth Factor (HBEGF))
- 别名
- Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF 产品)
- 别名
- hbegf CLIA Kit, zgc:165443 CLIA Kit, HBEGF CLIA Kit, zgc:175091 CLIA Kit, DTR CLIA Kit, DTS CLIA Kit, DTSF CLIA Kit, HEGFL CLIA Kit, Dtr CLIA Kit, GFHB CLIA Kit, Hb-egf CLIA Kit, Hegfl CLIA Kit, AW047313 CLIA Kit, Dts CLIA Kit, HB-EGF CLIA Kit, heparin-binding EGF-like growth factor b CLIA Kit, heparin binding EGF like growth factor CLIA Kit, Heparin-binding EGF-like growth factor CLIA Kit, heparin-binding EGF-like growth factor a CLIA Kit, heparin-binding EGF-like growth factor CLIA Kit, hbegfb CLIA Kit, HBEGF CLIA Kit, hbegf CLIA Kit, hbegfa CLIA Kit, Hbegf CLIA Kit
- 背景
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Gene Name: Heparin Binding Epidermal Growth Factor Like Growth Factor
Gene Aliases: HB-EGF, DTR, DTS, DTSF, HEGFL, Diphtheria Toxin Receptor, Proheparin-binding EGF-like growth factor, Diphtheria toxin receptor
- 途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
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