HBEGF ELISA 试剂盒
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- 抗原 See all HBEGF ELISA试剂盒
- HBEGF (Heparin-Binding EGF-Like Growth Factor (HBEGF))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 15.6 pg/mL - 1000 pg/mL
- 最低检测浓度
- 15.6 pg/mL
- 应用范围
- ELISA
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF). No significant cross-reactivity or interference between Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) and analogues was observed.
- 灵敏度
- 6.1 pg/mL
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Heparin-Binding EGF-Like Growth Factor (HBEGF) ELISA Kit
HBEGF 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Heparin-Binding EGF-Like Growth Factor (HBEGF) ELISA KitHBEGF 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Heparin-Binding EGF-Like Growth Factor (HBEGF) ELISA KitHBEGF 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Heparin-Binding EGF-Like Growth Factor (HBEGF) ELISA KitHBEGF 适用: 人 AA 63-148 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Heparin-Binding EGF-Like Growth Factor (HBEGF) ELISA KitHBEGF 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all HBEGF ELISA试剂盒
- HBEGF (Heparin-Binding EGF-Like Growth Factor (HBEGF))
- 别名
- Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF 产品)
- 别名
- hbegf ELISA Kit, zgc:165443 ELISA Kit, HBEGF ELISA Kit, zgc:175091 ELISA Kit, DTR ELISA Kit, DTS ELISA Kit, DTSF ELISA Kit, HEGFL ELISA Kit, Dtr ELISA Kit, GFHB ELISA Kit, Hb-egf ELISA Kit, Hegfl ELISA Kit, AW047313 ELISA Kit, Dts ELISA Kit, HB-EGF ELISA Kit, heparin-binding EGF-like growth factor b ELISA Kit, heparin binding EGF like growth factor ELISA Kit, Heparin-binding EGF-like growth factor ELISA Kit, heparin-binding EGF-like growth factor a ELISA Kit, heparin-binding EGF-like growth factor ELISA Kit, hbegfb ELISA Kit, HBEGF ELISA Kit, hbegf ELISA Kit, hbegfa ELISA Kit, Hbegf ELISA Kit
- 背景
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Gene Name: Heparin Binding Epidermal Growth Factor Like Growth Factor
Gene Aliases: HB-EGF, DTR, DTS, DTSF, HEGFL, Diphtheria Toxin Receptor, Proheparin-binding EGF-like growth factor, Diphtheria toxin receptor
- 基因ID
- 15200
- UniProt
- Q06186
- 途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
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