Growth Hormone 1 ELISA 试剂盒
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- 抗原 See all Growth Hormone 1 (GH1) ELISA试剂盒
- Growth Hormone 1 (GH1)
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Competition ELISA
- 检测范围
- 123.5 pg/mL - 10000 pg/mL
- 最低检测浓度
- 123.5 pg/mL
- 应用范围
- ELISA
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Growth Hormone (GH). No significant cross-reactivity or interference between Growth Hormone (GH) and analogues was observed.
- 灵敏度
- 46.2 pg/mL
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Growth Hormone 1 (GH1) ELISA Kit
GH1 适用: 小鸡 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum
Growth Hormone 1 (GH1) ELISA KitGH1 适用: 犬 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Growth Hormone 1 (GH1) ELISA KitGH1 适用: 犬 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum
Growth Hormone 1 (GH1) ELISA KitGH1 适用: Pig Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
Growth Hormone 1 (GH1) ELISA KitGH1 适用: Cow Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Growth Hormone 1 (GH1) ELISA KitGH1 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Growth Hormone 1 (GH1) ELISA KitGH1 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Growth Hormone 1 (GH1) ELISA KitGH1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Growth Hormone 1 (GH1) ELISA KitGH1 适用: Cow Colorimetric Competition ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Growth Hormone 1 (GH1) ELISA KitGH1 适用: 小鸡 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 2 h
- 板类型
- Pre-coated
- 实验流程
- This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Growth Hormone (GH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Growth Hormone (GH) and unlabeled Growth Hormone (GH) (Standards or samples) with the pre-coated antibody specific to Growth Hormone (GH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Growth Hormone (GH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Growth Hormone (GH) in the sample.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Growth Hormone (GH) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Growth Hormone (GH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all Growth Hormone 1 (GH1) ELISA试剂盒
- Growth Hormone 1 (GH1)
- 别名
- Growth Hormone (GH1 产品)
- 别名
- GH ELISA Kit, GH-N ELISA Kit, GHN ELISA Kit, IGHD1B ELISA Kit, hGH-N ELISA Kit, Gh1 ELISA Kit, GH1 ELISA Kit, gh ELISA Kit, Gh ELISA Kit, GH2 ELISA Kit, RNGHGP ELISA Kit, ghl ELISA Kit, gh-n ELISA Kit, ghn ELISA Kit, ighd1b ELISA Kit, gh1 ELISA Kit, ghb-A ELISA Kit, GHI ELISA Kit, GHB3 ELISA Kit, growth hormone 1 ELISA Kit, growth hormone ELISA Kit, somatotropin-like ELISA Kit, somatotropin ELISA Kit, growth hormone prepeptide ELISA Kit, growth hormone 1 L homeolog ELISA Kit, growth hormone 1 S homeolog ELISA Kit, Somatotropin-1 ELISA Kit, GH1 ELISA Kit, Gh ELISA Kit, GH ELISA Kit, LOC100305005 ELISA Kit, Gh1 ELISA Kit, LOC100534452 ELISA Kit, LOC100232594 ELISA Kit, LOC100303681 ELISA Kit, gh1 ELISA Kit, LOC100356068 ELISA Kit, LOC100136588 ELISA Kit, gh1.L ELISA Kit, gh1.S ELISA Kit, LOC109081196 ELISA Kit
- 背景
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Gene Name: Growth Hormone
Gene Aliases: GH1, GH-N, GHN, hGH-N, Somatotropin, Hygetropin, Jintropin, Kigtropin, Pituitary Growth Hormone, Growth Hormone, Normal
- 基因ID
- 14599
- UniProt
- P06880
- 途径
- NF-kappaB Signaling, JAK/STAT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Response to Growth Hormone Stimulus, Regulation of Hormone Biosynthetic Process
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