GATA3 ELISA 试剂盒
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- 抗原 See all GATA3 ELISA试剂盒
- GATA3 (GATA Binding Protein 3 (GATA3))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 31.25 pg/mL - 2000 pg/mL
- 最低检测浓度
- 31.25 pg/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of GATA Binding Protein 3 (GATA3). No significant cross-reactivity or interference between GATA Binding Protein 3 (GATA3) and analogues was observed.
- 灵敏度
- 11.8 pg/mL
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GATA Binding Protein 3 (GATA3) ELISA Kit
GATA3 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
GATA Binding Protein 3 (GATA3) ELISA KitGATA3 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
GATA Binding Protein 3 (GATA3) ELISA KitGATA3 适用: 人 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
GATA Binding Protein 3 (GATA3) ELISA KitGATA3 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Lysate, Tissue Homogenate
GATA Binding Protein 3 (GATA3) ELISA KitGATA3 适用: 人 Colorimetric DNA-Binding ELISA Cell Lysate, Nuclear Extract
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to GATA Binding Protein 3 (GATA3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to GATA Binding Protein 3 (GATA3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GATA Binding Protein 3 (GATA3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of GATA Binding Protein 3 (GATA3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GATA Binding Protein 3 (GATA3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GATA Binding Protein 3 (GATA3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all GATA3 ELISA试剂盒
- GATA3 (GATA Binding Protein 3 (GATA3))
- 别名
- GATA Binding Protein 3 (GATA3 产品)
- 别名
- HDR ELISA Kit, HDRS ELISA Kit, Gata-3 ELISA Kit, jal ELISA Kit, cb550 ELISA Kit, gta3 ELISA Kit, GATA-3 ELISA Kit, XGATA3 ELISA Kit, xgata-3 ELISA Kit, hdr ELISA Kit, xGATA-3 ELISA Kit, GATA3 ELISA Kit, GATA transcription factor 3 ELISA Kit, T4L20.260 ELISA Kit, T4L20_260 ELISA Kit, gata3 ELISA Kit, ZH-DR ELISA Kit, zgc:103531 ELISA Kit, GATA binding protein 3 ELISA Kit, GATA binding protein 3 S homeolog ELISA Kit, GATA transcription factor 3 ELISA Kit, amidase ELISA Kit, putative amidase ELISA Kit, GATA-binding protein 3 ELISA Kit, hematopoietic death receptor ELISA Kit, GATA3 ELISA Kit, Gata3 ELISA Kit, gata3 ELISA Kit, gata3.S ELISA Kit, gatA3 ELISA Kit, hdr ELISA Kit
- 背景
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Gene Name: GATA Binding Protein 3
Gene Aliases: HDR, Trans-acting T-cell-specific transcription factor GATA-3
- 途径
- Hormone Transport, Regulation of Hormone Metabolic Process, Tube Formation
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