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GATA3 ELISA 试剂盒

GATA3 适用: 人 Colorimetric DNA-Binding ELISA Cell Lysate, Nuclear Extract
产品编号 ABIN5690755
发货至: 中国
  • 抗原 See all GATA3 ELISA试剂盒
    GATA3 (GATA Binding Protein 3 (GATA3))
    适用
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    检测方法
    Colorimetric
    实验类型
    DNA-Binding ELISA
    应用范围
    ELISA
    原理
     Human GATA-3 Transcription Factor Activity Assay. This assay uses a dsDNA coated plate with canonical GATA3 binding sequences to semi-quantitatively detect active GATA3 in lysates or nuclear extracts. Only available in North America.
    样品类型
    Cell Lysate, Nuclear Extract
    Analytical Method
    Semi-Quantitative
    特异性
    The olionucleotide/antibody pair provided in this kit recognizes human GATA-3 in whole lysates and nuclear extracts.
    产品特性
    • Specific transcription factor-DNA binding assay
    • Perfect alternative to EMSA
    • Easy to perform in an ELISA format
    • Non-radioactive assay
    • High throughput (96 well plate format)
    • Assay can be completed within 5 hours
    组件
    • 96-well Strip Microplate pre-coated with DNA probes
    • DNA Binding Buffer
    • Positive Control Sample
    • Specific Competitor DNA probe
    • Non-specific Competitor DNA probe
    • Assay Reagent
    • DTT
    • Wash Buffer
    • Primary Antibody
    • HRP-conjugated Secondary Antibody
    • TMB One-Step Substrate Reagent
    • Stop Solution
    试剂未包括
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Absorbent paper
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
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  • 板类型
    Pre-coated
    实验流程
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared HRP-secondary antibody to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    4 °C
    储存方法
    Upon receipt, the positive control should be removed and stored at -20° or -80°C. The remainder of the kit can be stored for up to 6 months at 2-8°C from the date of shipment. Opened Microplate Wells or reagents may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: The kit can be used within one year if the whole kit is stored at -20°C upon receipt. Avoid repeated freeze-thaw cycles.
    有效期
    6 months
  • 抗原 See all GATA3 ELISA试剂盒
    GATA3 (GATA Binding Protein 3 (GATA3))
    别名
    GATA3 (GATA3 产品)
    别名
    HDR ELISA Kit, HDRS ELISA Kit, Gata-3 ELISA Kit, jal ELISA Kit, cb550 ELISA Kit, gta3 ELISA Kit, GATA-3 ELISA Kit, XGATA3 ELISA Kit, xgata-3 ELISA Kit, hdr ELISA Kit, xGATA-3 ELISA Kit, GATA3 ELISA Kit, GATA transcription factor 3 ELISA Kit, T4L20.260 ELISA Kit, T4L20_260 ELISA Kit, gata3 ELISA Kit, ZH-DR ELISA Kit, zgc:103531 ELISA Kit, GATA binding protein 3 ELISA Kit, GATA binding protein 3 S homeolog ELISA Kit, GATA transcription factor 3 ELISA Kit, amidase ELISA Kit, putative amidase ELISA Kit, GATA-binding protein 3 ELISA Kit, hematopoietic death receptor ELISA Kit, GATA3 ELISA Kit, Gata3 ELISA Kit, gata3 ELISA Kit, gata3.S ELISA Kit, gatA3 ELISA Kit, hdr ELISA Kit
    基因ID
    2625
    UniProt
    P23771
    途径
    Hormone Transport, Regulation of Hormone Metabolic Process, Tube Formation
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