Ephrin A5 CLIA Kit
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- 抗原 See all Ephrin A5 (EFNA5) CLIA Kits
- Ephrin A5 (EFNA5)
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适用
- 人
- 检测方法
- Chemiluminescent
- 实验类型
- Sandwich ELISA
- 检测范围
- 156.25 pg/mL - 10000 pg/mL
- 最低检测浓度
- 156.25 pg/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Ephrin A5 (EFNA5). No significant cross-reactivity or interference between Ephrin A5 (EFNA5) and analogues was observed.
- 灵敏度
- 6.52 pg/mL
- Top Product
- Discover our top product EFNA5 ELISA Kit
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Ephrin A5 (EFNA5) ELISA Kit
EFNA5 适用: 人 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Ephrin A5 (EFNA5) ELISA KitEFNA5 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 2 - 3 h
- 板类型
- Pre-coated
- 实验流程
- The microplate provided in this kit has been pre-coated with an antibody specific to Ephrin A5 (EFNA5). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Ephrin A5 (EFNA5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Ephrin A5 (EFNA5) level in the sample or standard.,
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ephrin A5 (EFNA5) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ephrin A5 (EFNA5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all Ephrin A5 (EFNA5) CLIA Kits
- Ephrin A5 (EFNA5)
- 别名
- Ephrin A5 (EFNA5 产品)
- 别名
- EFNA5 CLIA Kit, af1 CLIA Kit, efl5 CLIA Kit, rags CLIA Kit, eplg7 CLIA Kit, lerk7 CLIA Kit, AL-1 CLIA Kit, AV158822 CLIA Kit, EFL-5 CLIA Kit, Ephrin-A5 CLIA Kit, Epl7 CLIA Kit, LERK-7 CLIA Kit, RAGS CLIA Kit, Lerk7 CLIA Kit, AF1 CLIA Kit, EFL5 CLIA Kit, EPLG7 CLIA Kit, GLC1M CLIA Kit, LERK7 CLIA Kit, ephrin A5 CLIA Kit, EFNA5 CLIA Kit, efna5 CLIA Kit, Efna5 CLIA Kit
- 背景
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Gene Name: Ephrin A5
Gene Aliases: AF1, EFL5, EPLG7, GLC1M, LERK7, RAGS, AL-1, EPH-related receptor tyrosine kinase ligand 7
- 基因ID
- 1946
- UniProt
- P52803
- 途径
- RTK signaling
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