Endonuclease G ELISA 试剂盒
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- 抗原 See all Endonuclease G (ENDOG) ELISA试剂盒
- Endonuclease G (ENDOG)
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适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 78.12 pg/mL - 5000 pg/mL
- 最低检测浓度
- 78.12 pg/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Endonuclease G, Mitochondrial (ENDOG). No significant cross-reactivity or interference between Endonuclease G, Mitochondrial (ENDOG) and analogues was observed.
- 灵敏度
- 32 pg/mL
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Endonuclease G (ENDOG) ELISA Kit
ENDOG 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Endonuclease G (ENDOG) ELISA KitENDOG 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Endonuclease G (ENDOG) ELISA KitENDOG 适用: 大鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Lysate, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Endonuclease G, Mitochondrial (ENDOG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Endonuclease G, Mitochondrial (ENDOG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Endonuclease G, Mitochondrial (ENDOG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Endonuclease G, Mitochondrial (ENDOG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Endonuclease G, Mitochondrial (ENDOG) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Endonuclease G, Mitochondrial (ENDOG) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all Endonuclease G (ENDOG) ELISA试剂盒
- Endonuclease G (ENDOG)
- 别名
- Endonuclease G, Mitochondrial (ENDOG 产品)
- 别名
- CG8862 ELISA Kit, Dmel\\CG8862 ELISA Kit, Tb08.10J17.140 ELISA Kit, Tb08.10J17.90 ELISA Kit, TBC1D13 ELISA Kit, endog ELISA Kit, ENDOG ELISA Kit, wu:fb79c08 ELISA Kit, zgc:110020 ELISA Kit, Endonuclease G ELISA Kit, endonuclease G ELISA Kit, putative endonuclease G ELISA Kit, endonuclease G, mitochondrial ELISA Kit, endonuclease G L homeolog ELISA Kit, EndoG ELISA Kit, Tc00.1047053506867.10 ELISA Kit, Tb927.8.4040 ELISA Kit, Tb927.8.4090 ELISA Kit, LBRM_10_0750 ELISA Kit, LMJF_10_0610 ELISA Kit, NAEGRDRAFT_78507 ELISA Kit, ENDOG ELISA Kit, endog ELISA Kit, eNDOG ELISA Kit, LOC100529218 ELISA Kit, Endog ELISA Kit, endog.L ELISA Kit
- 背景
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Gene Name: Endonuclease G, Mitochondrial
Gene Aliases: ENDO-G
- 基因ID
- 362100
- UniProt
- Q3V5X8
- 途径
- Apoptosis
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